Person:
KAYA, ÖZLEM TUĞÇE

Loading...
Profile Picture

Email Address

Birth Date

Research Projects

Organizational Units

Organizational Unit

Job Title

Last Name

KAYA

First Name

ÖZLEM TUĞÇE

Name

Search Results

Now showing 1 - 10 of 27
  • PublicationOpen Access
    Ghrelin Treatment Improves Lipid Metabolism and Hepatic Degeneration in Ovariectomized Rats
    (GAZI UNIV, FAC MED, 2020-01-01) YEGEN, BERRAK; Gurler, Esra Bihter; Ozbeyli, Dilek; Kaya, Ozlem Tugce Cilingir; Ercan, Feriha; Yegen, Berrak C.
    Objective: Metabolic disorders occurring in post-menopausal period increase the risk for development of fatty liver disease in women. Aim of the study was to evaluate possible effects of ghrelin on metabolic biomarkers and hepatic morphology in ovariectomized (OVT) rats. Methods:Under ketamine-chlorpromazine anesthesia (100 mg/kg, 0.75 mg/kg), Sprague-Dawley rats (n=12) underwent bilateral OVT, while control group had sham-surgery (n=6). Four weeks after surgery, half of OVT rats were treated intraperitonally with ghrelin (1 mg/kg/hafta) for 4 weeks, while others were not treated. Rats were euthanized by cardiac puncture at the end of 8th weeks, and serum levels of glucose, insulin, aspartate aminotransferase (AST), high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), triglycerides, estradiol and progesterone were measured by an automated analyzer. Results: Increased body weights in OVT rats (p<0.001) recorded at the end of 2 months was not changed with ghrelin. Serum estradiol and progesterone levels were reduced (p<0.05) verifying altered gonadal hormone status, but insulin and glucose levels were not changed. Reduced HDL and increased LDL levels (p<0.0.5) were evident in non-treated OVX rats, while ghrelin treatment depressed LDL levels (p<0.0.5), but did not change HDL levels. However, ghrelin in OVT rats depressed triglycerides, VLDL and AST levels significantly (p<0.05). Moderate sinusoidal congestion, activated Kupffer cells and hepatocytes with ballooning degeneration was observed in non-treated OVT rats, while significant improvements were present in livers of ghrelin-treated rats. Conclusion: In conclusion, mild dyslipidemia and hepatic degeneration in early post-menopausal period appear to be attenuated by ghrelin treatment, and require further investigation.
  • Publication
    Nesfatin-1 treatment preserves antioxidant status and attenuates renal fibrosis in rats with unilateral ureteral obstruction
    (2022-06-01) ÇETİNEL, ŞULE; YEGEN, BERRAK; KAYA, ÖZLEM TUĞÇE; ÖZBEYLİ, DİLEK; Tezcan N., Özdemir-Kumral Z. N., Yenal N. Ö., Çilingir-Kaya Ö. T., Virlan A. T., Özbeyli D., Çetinel Ş., Yeğen B., Koç M.
    Background Nesfatin-1 (NES-1), an anorexigenic peptide, was reported to have anti-inflammatory and anti-apoptotic actions in several inflammation models. Methods To elucidate potential renoprotective effects of NES-1, unilateral ureteral obstruction (UUO) was induced in male Sprague Dawley rats by ligating left ureters. The rats were injected intraperitoneally with either saline (SL) or NES-1 (10 mu g/kg/day) for 7 or 14 days (n = 8 in each group). On the 7th or 14th day, obstructed kidneys were removed for the isolation of leucocytes for flow-cytometric analysis and the assessments of biochemical and histopathological changes. Results Opposite to glutathione levels, renal myeloperoxidase activity in the SL-treated UUO group was significantly increased compared with the sham-operated group, while NES-1 treatment abolished the elevation. The percentages of CD8+/CD4+ T-lymphocytes infiltrating the obstructed kidneys were increased in the SL-treated groups but treatment with NES-1 did not prevent lymphocyte infiltration. Elevated tumour necrosis factor-alpha (TNF-alpha) levels in SL-treated UUO group were decreased with NES-1. Although total degeneration scores were similarly increased in all UUO groups, tubular dilatation scores were significantly increased in UUO groups and lowered by NES-1 only in the 7-day treated group. Elevated interstitial fibrosis scores in the SL-treated groups were decreased in both 7- and 14-day NES-1 treated groups, while alpha-smooth muscle actin (alpha-SMA) and apoptosis scores were depressed in both NES-1 treated groups. Conclusion The present data demonstrate that UUO-induced renal fibrosis is ameliorated by NES-1, which appears to involve the inhibition of neutrophil infiltration and thereby amelioration of oxidative stress and inflammation. These data suggest that NES-1 may have a regulatory role in protecting the kidneys against obstruction-induced renal injury.
  • PublicationOpen Access
    Investigation of neurogenesis in kindled wistar and genetic absence epilepsy rats
    (2022-09-01) ŞİRVANCI, SERAP; KAYA, ÖZLEM TUĞÇE; Kandemir C., Yavuz M., Karakaya F. B. , Kaya Ö. T. , Onat F., Şirvancı S.
  • Publication
    Primer siliyer diskinezi tanısında transmisyon elektron mikroskopi
    (Türkiye Klinikleri, 2022-01-01) KAYA, ÖZLEM TUĞÇE; ŞİRVANCI, SERAP; ŞİRVANCI S., KAYA Ö. T.
    Primer siliyer diskinezi (PSD), siliya fonksiyon bozukluğunun neden olduğu, çoğunlukla otozomal resesif geçişli, klinik ve genetik olarak heterojen bir hastalıktır. PSD klinik fenotipi değişkendir. Üst ve alt solunum sistemi, üreme organları, kalp ve siliyaların bulunduğu diğer sistemleri tutar. Zamanında doğan PSD'li bebeklerin yaklaşık %80'i, doğum sonrası 24 saat içinde solunum sıkıntısı kliniği gösterir ve oksijen desteği gerektirir. Sonraki dönemde tekrarlayan pnömoni veya bronşit yaygın görülür. Kronik, efüzyonlu tekrarlayan orta kulak iltihabı, özellikle yaşamın ilk yılında, PSD'li çocukların en az %80'ini etkiler. Hastaların yaklaşık %80'inde yıl boyu devam eden burun tıkanıklığı ve kronik sinüzit vardır. Situs inversus totalis daha sık olmak üzere bir dizi organ lateralite kusuru görülür. Erkeklerde infertilite, kadınlarda ektopik gebelik sıktır. Bazı durumlarda, PSD'nin polikistik böbrek, hidrosefali, polispleni gibi nadir ve olağandışı bozukluklarla ilişkili olduğu gösterilmiştir. Son zamanlarda genetik çalışmaların hızlanmasıyla yapılan çalışmalar, farklı genlerdeki mutasyonların değişken fenotiplere yol açtığını göstermektedir. Bununla ilgili vaka serileri mevcuttur.
  • PublicationOpen Access
    Effect of perinatal and postnatal thiamine deficiency on auditory pathway of the Wistar-Albino rats
    (2022-01-01) KAYA, ÖZLEM TUĞÇE; ŞİRVANCI, SERAP; Gür Ö. E., Yılmaz N. D. S., Ensari N., Senirli R. T., GÜLMEZ Z. D., KAYA Ö. T., ŞİRVANCI S., Danışman B., DERİN N., Yılmaz M. D.
    © 2022 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-FacialObjective: In this study, we created an animal model to demonstrate the effects of thiamine on the hearing pathways of new-borns during pregnancy and lactation by inducing a dietary thiamine deficiency in the mother. Methods: The study included 16 female Wistar albino rats. The animals were separated into four groups and provided the appropriate amounts of dietary thiamine according to their groups during pre-pregnancy, pregnancy, and lactation periods. Three pups from each mother were included in the study, and 12 pups were selected from each group. On the fortieth day after birth, the auditory pathways of 48 pups in the 4 groups were examined electro physiologically and ultra-structurally. Results: In Group N-N, morphology of hair cells stereocilia degeneration was not obtained in all turns of cochlea. In Group N-T, Inner Hair Cells (IHCs) and Outher Hair Cells (OHCs) stereocilia didn\"t show degeneration in all turns of cochlea but had rupture inrows of HCs stereocilia. In group T-N IHCs stereocilia less degeneration was observed in all turns of cochlea. OHC stereocilia partial loss was observed only in basal turn of cochlea. In Group T-T IHCs stereocilia was observed less degeneration and rupture in all turns of cochlea. Conclusion: Thiamine is vital for the development of cochlear hair cells during both prenatal and postnatal periods. Even partial deficiency of thiamine causes significant degeneration to the auditory pathway. Level of evidence: The level of evidence of this article is 5. This article is an experimental animal and laboratory study.
  • Publication
    Oral histoloji
    (Quintessence Publishing Co, Inc., 2023-12-01) KAYA, ÖZLEM TUĞÇE; Kaya Ö. T. (Editör), Gürler E. B. (Editör), Aydın M. Ş. (Editör)
  • PublicationOpen Access
    The effect of topical and systemic tranexamic acid on fracture healing in rats
    (TURKISH ASSOC ORTHOPAEDICS TRAUMATOLOGY, 2020-04-03) ERCAN, FERİHA; Cevik, Huseyin Bilgehan; Eceviz, Engin; Kaya, Ozlem Tugce Cilingir; Ercan, Feriha; Cecen, Gultekin Sitki
    Objective: The aim of the present study was to determine the effect of topical and systemic tranexamic acid (TXA) on fracture healing in a rat surgical model. Methods: We created standard, right-sided, open, diaphyseal femoral fractures with intramedullary Kirschner wire fixation in 48 male rats and divided them into three groups: a topical TXA (10 mg/kg) group, a systemic TXA (10 mg/kg) group, and a control group. Fracture healing was evaluated radiographically and histologically after early (week 2) and late (week 4) postoperative sacrifice. Results: The radiological scores differed significantly among the all groups (p-0.001), as did the week 2 and 4 scores (p=0.003 and p=0.010, respectively). Radiologically, the topical TXA group exhibited better bone healing at both 2 (p=0.001) and 4 (p=0.007) weeks than the control group, and the systemic group showed better healing at both 2 (p=0.027) and 4 (p=0.023) weeks than the control TXA group. Moreover, bone healing was better in the group treated with topical rather than systemic TXA on radiological examinations performed at 2 (p=0.001) and 4 (p=0.007) weeks postoperatively (p=0.001 and p=0.007, respectively). Histologically, the groups differed significantly (p=0.001). The histological scores differed significantly among the all groups (p=0.001). At 2 weeks, the topical TXA group exhibited significantly better bone healing than the control group (p=0.001). Conclusion: Our results suggested that topical application of TXA in fracture patients may accelerate healing, whereas systemic administration may adversely affect healing.
  • Publication
    Ultrastructural investigation of synaptic alterations in the rat hippocampus after irradiation and hyperthermia
    (TAYLOR & FRANCIS INC, 2020) ERCAN, FERİHA; Erkanli Senturk, Gozde; Cilingir-Kaya, Ozlem Tugce; Sirvanci, Serap; Isler, Cihan; Kemerdere, Rahsan; Ulu, Mustafa Onur; Umay, Cenk; Onat, Filiz; Ozkara, Cigdem; Uzan, Mustafa; Ercan, Feriha
    This study aimed to investigate ultrastructural synaptic alterations in rat hippocampus after in utero exposure to irradiation (IR) and postnatal exposure to hyperthermia (HT). There were four groups in each of the time points (3(rd) and 6(th) months). IR group: Pregnant rats were exposed to radiation on the 17(th) gestational day. HT group: Hyperthermia was applied to the rat pups on the 10th day after their birth. IR+HT group: Both IR and HT were applied at the same time periods. Control group: No IR or HT was applied. Rat pups were sacrificed after 3 and 6 months. Thin sections from the dentate gyrus (DG) and the CA3 of hippocampus were evaluated for synapse numbers by electron microscopy. Synapses were counted, and statistical analysis was performed. Abnormalities in myelin sheath, mossy terminals and neuropil were observed in the CA3 and DG of all groups. The synapses in the CA3 region were significantly increased in the IR-3(rd) month, IR-6(th) month, and IR+HT-3(rd) month groups vs control group. Synapses were significantly increased in the DG of HT-3(rd) month group. A trend for an increase in synapse numbers was seen in the CA3 and DG. Increased number of synapses in the rat hippocampus may be due to mossy fiber sprouting, possibly caused by in utero irradiation and/or postnatal hyperthermia.
  • PublicationOpen Access
    The protective effects of momordica charantia fruit extractin methotrexate induced liver damage in rats
    (2022-12-01) ÖZBEYLİ, DİLEK; KAYA, ÖZLEM TUĞÇE; Özbeyli D., Şen A., Çevik Ö., Erdoğan Ö., Kaya Ö. T., Ede Pazarbaşı S., Şener G.
    BACKGROUND/AIMS: Methotrexate (MTX), a cytotoxic therapeutic agent, is used for the cure of malignancies and rheumatologic disorders. However, the significant side effects of MTX limits its use. In this study, we aim to assess the hepatoprotective properties of Momordica charantia (MC) against MTX-induced liver damaged in rats. MATERIALS AND METHODS: Following one dose of MTX (20 mg/kg), the rats were given either distilled water or MC extract (300 mg/kg, po) for 5 days. After the dissection of the rats, the liver was removed to analyse tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β), transforming growth factor β (TGF-β) and 8-hydroxy-2’-deoxy-guanosine (8-OhdG) levels and superoxide dismutase (SOD), catalase (CAT), and caspase-3 activities. The tissues were also examined histopathologically. RESULTS: The hepatic TNF-α, IL-1β, TGF-β, 8-OhdG levels, and Caspase-3 activity in the MTX group were found to be significantly increased compared to the control group. However, MC extract was able to significantly decrease TNF-α, TGF-β, 8-OhdG levels, and Caspase-3 activity. Also, both the SOD and CAT activity of the MTX group decreased compared to the control group. Although only the SOD levels elevated significantly with MC treatment, the SOD and CAT activities of the MC treated group were similar to the control group. Supporting these biochemical parameters, MTX-induced histologic alterations in the liver were also ameliorated via MC treatment. CONCLUSION: Our results demonstrated that MC has a protective role against MTX-induced hepatic tissue injury by reducing apoptosis, oxidative damage, and the expression of pro-inflammatory cytokines.
  • Publication
    Effect of Tacrolimus on Peripheral Nerve Regeneration in Allograft Transplantation: A Light and Electron Microscopic Study
    (2021) KAYA, ÖZLEM TUĞÇE; Cilingir-Kaya, Ozlem Tugce; Sumer, Onur; Sirvanci, Serap; Gurler, Esra Bihter; Akcal, Arzu; Karsidag, Semra
    OBJECTIVES: Peripheral nerve injuries are common in Europe; however, the treatment techniques may lead to disabilities. This study aimed to evaluate the effect of tacrolimus use on the capacity of the epineural sheath graft to improve its regeneration quality in rat sciatic nerves as a treatment option for nerve injuries. MATERIALS AND METHODS: In the experimental process, 30 male Sprague Dawley were used as recipients and 10 Wistar rats were used as donors. Under anesthesia, all rats were operated on to resect the sciatic nerve. The nerve tissue of Wistar rats was used as allograft. In the autograft group, the resected nerve was reversed and sutured, resulting in an epineural sheath graft. For the allograft groups, rats were randomly divided into 2 groups as the tacrolimus-treated group and the nontreated group after allograft transplant. Tacrolimus was administered intramuscularly at 0.1 mg/kg daily for 12 weeks. After the treatment period, rats were killed and evaluated histomorphologically with light and electron microscopy. RESULTS: Histological examination showed no remarkable differences between different regions of the sciatic nerves (distal, middle, and proximal). The axonal density was decreased in the allograft groups compared with the autograft group (P < .001). Results showed that the number of mast cells was increased in the allograft group without tacrolimus treatment (P < .05). Similarly, there was a mild increase in mast cell count in the tacrolimus-treated allograft group. CONCLUSIONS: Our results showed that tacrolimus use in rats with implanted epineural nerve sheath supported recovery in terms of morphological and physiological regeneration of the nerve.