Publication: Nesfatin-1 treatment preserves antioxidant status and attenuates renal fibrosis in rats with unilateral ureteral obstruction
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2022-06-01
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Abstract
Background Nesfatin-1 (NES-1), an anorexigenic peptide, was reported to have anti-inflammatory and anti-apoptotic actions in several inflammation models. Methods To elucidate potential renoprotective effects of NES-1, unilateral ureteral obstruction (UUO) was induced in male Sprague Dawley rats by ligating left ureters. The rats were injected intraperitoneally with either saline (SL) or NES-1 (10 mu g/kg/day) for 7 or 14 days (n = 8 in each group). On the 7th or 14th day, obstructed kidneys were removed for the isolation of leucocytes for flow-cytometric analysis and the assessments of biochemical and histopathological changes. Results Opposite to glutathione levels, renal myeloperoxidase activity in the SL-treated UUO group was significantly increased compared with the sham-operated group, while NES-1 treatment abolished the elevation. The percentages of CD8+/CD4+ T-lymphocytes infiltrating the obstructed kidneys were increased in the SL-treated groups but treatment with NES-1 did not prevent lymphocyte infiltration. Elevated tumour necrosis factor-alpha (TNF-alpha) levels in SL-treated UUO group were decreased with NES-1. Although total degeneration scores were similarly increased in all UUO groups, tubular dilatation scores were significantly increased in UUO groups and lowered by NES-1 only in the 7-day treated group. Elevated interstitial fibrosis scores in the SL-treated groups were decreased in both 7- and 14-day NES-1 treated groups, while alpha-smooth muscle actin (alpha-SMA) and apoptosis scores were depressed in both NES-1 treated groups. Conclusion The present data demonstrate that UUO-induced renal fibrosis is ameliorated by NES-1, which appears to involve the inhibition of neutrophil infiltration and thereby amelioration of oxidative stress and inflammation. These data suggest that NES-1 may have a regulatory role in protecting the kidneys against obstruction-induced renal injury.
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Tıp, Dahili Tıp Bilimleri, İç Hastalıkları, Nefroloji, Sağlık Bilimleri, Medicine, Internal Medicine Sciences, Internal Diseases, Nephrology, Health Sciences, TRANSPLANTASYON, Klinik Tıp, Klinik Tıp (MED), ÜROLOJİ VE NEFROLOJİ, TRANSPLANTATION, CLINICAL MEDICINE, Clinical Medicine (MED), UROLOGY & NEPHROLOGY, Üroloji, Transplantasyon, Urology, Transplantation, apoptosis, inflammation, nesfatin-1, oxidative stress, unilateral ureteral obstruction, BLOOD-BRAIN-BARRIER, PROGRESSIVE FIBROSIS, EXPRESSION, INJURY, NEPHROPATHY, DAMAGE, ANTAGONIST, APOPTOSIS, BLOCKADE
Citation
Tezcan N., Özdemir-Kumral Z. N., Yenal N. Ö., Çilingir-Kaya Ö. T., Virlan A. T., Özbeyli D., Çetinel Ş., Yeğen B., Koç M., "Nesfatin-1 treatment preserves antioxidant status and attenuates renal fibrosis in rats with unilateral ureteral obstruction", NEPHROLOGY DIALYSIS TRANSPLANTATION, cilt.37, sa.7, ss.1238-1248, 2022