Person: SESAL, NÜZHET CENK
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SESAL
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NÜZHET CENK
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Publication Open Access Using proteomics, q-PCR and biochemical methods complementing as a multiapproach to elicit the crucial responses of zebrafish liver exposed to neonicotinoid pesticide(2023-06-01) SESAL, NÜZHET CENK; GÖKALSIN, BARIŞ; KAYHAN, FİGEN ESİN; Kızılkaya Ş., Akpınar G., Sesal N. C., Kasap M., Gökalsın B., Kayhan F. E.Pesticides enter the environment through runoff and leaching and this raises public concern about effects on non-target organisms. Imidacloprid (IMI) a synthetic pesticide, has an unstable half-life, metabolized in minutes to weeks in the water. To evaluate the effects of IMI on the zebrafish liver, we conducted proteomic, molecular and biochemical analysis in a multi-level approach, to highlight the complementary features regarding the results of each method. Adult zebrafish were exposed to 60 mg/L IMI for 48 hours and were evaluated using nLC-MS/MS for proteins, q-PCR analysis for expression of cat, gpx, pxr, ache, along with CAT and AChE enzyme activities and GSH and MDA assays. Based on proteomics, the regulation of antioxidant and immune responses, as well as gene transcription were significant processes affected. Apoptosis and ER stress pathways were upregulated and there was a down-regulation of cat and gpx genes. There was also elevated CAT activity and GSH and decreased MDA. Additionally, elevated AChE activity and up regulation of ache expression was observed. The multi-approach results included regulators of antioxidant, xenobiotic response and neuro-protective related proteins (genes and enzymes), which overall reflected harmful effects of IMI. Consequently, this study highlights the effects of IMI on zebrafish liver and reveals new potential biomarkers. In this respect, evaluated outcomes reveal the complementary features emphasizing the importance of studying chemicals using several methods. Our study provides deeper insights for future work in ecotoxicological studies regarding IMI and contribute to existing toxicity literature.Publication Open Access Effects of UV-B Radiation on the Performance, Antioxidant Response and Protective Compounds of Hazelnut Pollen(2022-10-01) ÇETİNBAŞ GENÇ, ASLIHAN; SESAL, NÜZHET CENK; ÇETİNBAŞ GENÇ A., Toksoz O., Piccini C., Kilin O., SESAL N. C. , Cai G.Increasing ultraviolet (UV) radiation is expected to become a problem in hazelnut cultivation. The aim of this study is to examine the effects of UV-B on hazelnut pollen. To this end, the pollens were exposed to UV-B for 1, 2, and 3 h at distances of 10, 20, 30, and 40 cm. Groups treated for 2 h at 20 cm and 3 h at 10 and 20 cm were identified as the most affected based on the results of viability, germination, and tube elongation. Further studies on these groups showed that UV-B does not change the DPPH radical scavenging activity for all groups. However, total phenolic compounds decreased after 3 h of treatment at 10 and 20 cm, while total flavonoid compounds decreased after all treatment groups. The UV-B absorbance of cytoplasmic and cell-wall-bound fractions decreased for all groups. The UV-B absorbance of the sporopollenin-derived fraction increased after 2 h of treatment at 20 cm but decreases after treatment for 3 h at 10 and 20 cm. In summary, exposure to UV-B for different times and distances adversely affected pollen grains in terms of pollen viability, germination rate, tube length, and the level of antioxidant molecules and UV-absorbing compounds.Publication Open Access An acetonic extract and secondary metabolites from the endolichenic fungus Nemania sp. EL006872 exhibit immune checkpoint inhibitory activity in lung cancer cell(2022-09-08) SESAL, NÜZHET CENK; Varlı M., Pham H. T. , Kim S., Taş İ., Gamage C. D. B. , Zhou R., Pulat S., Park S., SESAL N. C. , Hur J., et al.Copyright © 2022 Varlı, Pham, Kim, Taş, Gamage, Zhou, Pulat, Park, Sesal, Hur, Kang and Kim.Background: Endolichenic fungi (ELF), which live the inside the lichen thallus, contain many secondary metabolites that show various biological activities. Recent studies show that lichen and ELF secondary metabolites have antioxidant, antibacterial, antifungal, cytotoxic, and anticancer activities. Purpose: Here, the effects of an ELF extract and its bioactive compounds were investigated on the H1975 cell line focusing on immune checkpoint marker inhibition. Methods: An ELF was isolated from the host lichen Bryoria fuscescens (Gyelnik) Brodo and D. Hawksw and identified the species as Nemania sp. EL006872. The fungus was cultured on agar medium and acetonic extracts were obtained. Secondary metabolites radianspenes C and D, and dahliane D, were isolated from the crude extract. The biological effects of both the crude extract and the isolated secondary metabolites were evaluated in cell viability, qRT-PCR assays, flow cytometry analysis and western blotting. Results: The cell viability assay revealed that extracts from Nemania sp. EL006872 and the isolated secondary compounds had low cytotoxicity. The crude extract, radianspenes C and D, and dahliane D, suppressed expression of mRNA encoding PD-L1 and aromatic hydrocarbon receptor (AhR), and surface expression of PD-L1 protein by cells exposed to benzo[a] pyrene. Radianspenes C and D, and dahliane D, reduced expression of AhR, PD-L1, ICOSL, and GITRL proteins by H1975 lung cancer cells, as well as exerting anti-proliferative effects. Conclusion: Radianspenes C and D, and dahliane D, bioactive compounds isolated from Nemania sp. EL006872 ELF, have the potential for use as immunotherapy and immunoncology treatments.Publication Open Access Effects of ultrasonic specific energy on time-dependent inactivation rate of proteus mirabilis and streptococcus sanguinis(2023-01-01) GÖKALSIN, BARIŞ; SESAL, NÜZHET CENK; Kekeç Ö., GÖKALSIN B., Açıkgöz B., Özdaş D. Ö., Sesal C.Ultrasound has been used as an alternative and enhancer approach for the inhibition of microorganisms in recent years. The purpose of the current study was to determine the inactivation of Streptococcus sanguinis and Proteus mirabilis bacteria species via ultrasound. For this purpose, bacterial suspensions with 5x103, 1.5x104, and 3x104 colony forming unit (cfu/ml) concentrations were ultra-sonicated with 30 kHz frequency and 100 W power ultrasound for various sonication time periods. As a result, time-dependent inhibition rates were observed with bacteria concentration differences. The specific energy (γ) of different concentrations of S. sanguinis and P. mirabilis was also calculated. The rate constant of ultrasonic inhibition was evaluated in the linear region against sonication time. Then, the t-test was applied to all of the data. The results of 5x103, 1.5x104, and 3x104 cfu/ml for S. sanguinis and P. mirabilis were statistically significant atPublication Metadata only Could insects be an alternative food source? A comprehensive review(2024-04-01) TURAN, YAVUZ; SESAL, NÜZHET CENK; Turan Y., Berber D., Sesal N. C.According to the United Nations, more than 800 million people are exposed to starvation. It is predicted that the world population will face much more serious starvation for reasons such as global warming, diseases, economic problems, rapid urbanization, and destruction of agricultural areas and water resources. Thus, there are significant hesitations about the sustainability of food resources, and the search for alternative food sources has increased. One of the leading alternative food sources is insects. Although the use of edible insects has been accepted in some areas of the world, entomophagy is not preferred in some countries due to sociocultural conditions, health concerns, neophobia, and entomophobia. Many people do not accept the direct consumption of raw insects, but insects can be transformed into more preferred forms by using different cooking techniques. Some ground edible insects are satisfactory in terms of nutritional value and have a reasonable level of acceptability when added to products such as bread, tortilla, and pasta in varying percentages. The world market value of edible insects was estimated to be US$3.2 million in 2021 and US$17.6 billion in 2032. In this review, the current and future situation of insects as an alternative food source is comprehensively discussed.