Publication: Using proteomics, q-PCR and biochemical methods complementing as a multiapproach to elicit the crucial responses of zebrafish liver exposed to neonicotinoid pesticide
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Date
2023-06-01
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Abstract
Pesticides enter the environment through runoff and leaching and this raises public concern about effects on non-target organisms.
Imidacloprid (IMI) a synthetic pesticide, has an unstable half-life, metabolized in minutes to weeks in the water. To evaluate the effects
of IMI on the zebrafish liver, we conducted proteomic, molecular and biochemical analysis in a multi-level approach, to highlight the
complementary features regarding the results of each method. Adult zebrafish were exposed to 60 mg/L IMI for 48 hours and were
evaluated using nLC-MS/MS for proteins, q-PCR analysis for expression of cat, gpx, pxr, ache, along with CAT and AChE enzyme
activities and GSH and MDA assays. Based on proteomics, the regulation of antioxidant and immune responses, as well as gene
transcription were significant processes affected. Apoptosis and ER stress pathways were upregulated and there was a down-regulation
of cat and gpx genes. There was also elevated CAT activity and GSH and decreased MDA. Additionally, elevated AChE activity and
up regulation of ache expression was observed. The multi-approach results included regulators of antioxidant, xenobiotic response and
neuro-protective related proteins (genes and enzymes), which overall reflected harmful effects of IMI. Consequently, this study
highlights the effects of IMI on zebrafish liver and reveals new potential biomarkers. In this respect, evaluated outcomes reveal the
complementary features emphasizing the importance of studying chemicals using several methods. Our study provides deeper insights
for future work in ecotoxicological studies regarding IMI and contribute to existing toxicity literature.
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Yaşam Bilimleri, Hidrobiyoloji, Moleküler Biyoloji ve Genetik, Temel Bilimler, Life Sciences, Hydrobiology, Molecular Biology and Genetics, Natural Sciences, Temel Bilimler (SCI), Yaşam Bilimleri (LIFE), Doğa Bilimleri Genel, Biyoloji ve Biyokimya, ÇOK DİSİPLİNLİ BİLİMLER, BİYOLOJİ, Natural Sciences (SCI), Life Sciences (LIFE), NATURAL SCIENCES, GENERAL, BIOLOGY & BIOCHEMISTRY, MOLECULAR BIOLOGY & GENETICS, MULTIDISCIPLINARY SCIENCES, BIOLOGY, Moleküler Biyoloji, Multidisipliner, Molecular Biology, Multidisciplinary
Citation
Kızılkaya Ş., Akpınar G., Sesal N. C., Kasap M., Gökalsın B., Kayhan F. E., "Using proteomics, q-PCR and biochemical methods complementing as a multiapproach to elicit the crucial responses of zebrafish liver exposed to neonicotinoid pesticide", COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY - PART D: GENOMICS AND PROTEOMICS, cilt.101103, sa.101103, ss.101103, 2023