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OKTAY, NİHAL ŞEHKAR

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NİHAL ŞEHKAR

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2017 - 201912020 - 20232
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    PublicationOpen Access
    Comparison of the protective effect of alpha lipoic acid and quercetin in methotrexate- induced lung damage
    (2023-03-01) AK, ESİN; OKTAY, NİHAL ŞEHKAR; AK E., MUHAN A., ÇALIŞKAN Ş., OKTAY N. Ş.
    Objective: The aim of this experiment is to investigate and compare the effects of alpha lipoic acid (ALA) and quercetin (QUE) on methotrexate (MTX)-induced lung injury in rats. Method: Wistar Albino rats were distributed into control, MTX, MTX+ALA and MTX+QUE groups with each consisting of 6 rats. Except control group, MTX administrated to rats as a single dose (20 mg/kg) intraperitoneally (i.p.) on the first day. Saline (0.1 cc/100 gr/day, i.p.) was injected to rats in control and MTX groups for 5 days. In MTX+ALA and MTX+QUE groups, rats had injections of ALA (50 mg/kg/day, i.p.) and QUE (50 mg/kg/day, i.p.) for 5 days. After sacrification on day 6, lung tissues were excised out for histopathologic and biochemical investigation. Results: MTX group showed massive hemorrhage with edema in the interstitium, significant inflammatory cell infiltration, and severe alveolar destruction and vascular congestion. Additionally, significant increases in oxidative stress markers as malondialdehyde and sialic acid and significant decreases in antioxidants as glutathione, superoxide dismutase and catalase were detected at the tissue level in MTX group (p<0.0001, p<0.0001, p<0.0001, p=0.03 and p<0.0001, respectively). Both ALA and QUE treatment led to a prominent improvement in morphologic damage. Moreover, ALA and QUE resulted in the reversal of the alterations seen in the tissue oxidative damage markers and antioxidant activities as well. We could not reveal a significant difference between MTX+ALA and MTX+QUE group in terms of morphologic damage and biochemical markers of oxidative injury (p>0.05). Conclusion: Our study showed the similar protective effect of ALA and QUE in MTX induced lung damage. Further studies are warranted to verify the results of our outcome.
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    PublicationOpen Access
    Investigation of the Effects of Edaravone on Valproic Acid Induced Tissue Damage in Pancreas
    (MARMARA UNIV, FAC PHARMACY, 2017-06-20) YARAT, AYŞEN; Oktay, Sehkar; Alev-Tuzuner, Burcin; Tunali, Sevim; Ak, Esin; Emekli-Alturfan, Ebru; Tunali-Akbay, Tugba; Koc-Ozturk, Leyla; Cetinel, Sule; Yanardag, Refiye; Yarat, Aysen
    Valproic acid (VPA), an effective antiepileptic and anticonvulsant drug, has some toxic side effects due to causing elevated oxidant production. The aim of this study is to investigate the effects of edaravone, a potent free radical scavenger on VPA induced toxicity and tissue damage by biochemical and histological examinations on pancreas. Female Sprague Dawley rats were divided into four groups as follows; control, edaravone, VPA, VPA+edaravon. VPA and edaravone were injected intraperitonally for seven days. Total protein, lipid peroxidation (LPO), sialic acid (SA) and glutathione (GSH) levels and alkaline phosphatase (ALP), tissue factor (TF), superoxide dismutase (SOD), glutathione-S-transferase GST), catalase (CAT), glutathione peroxidase (GPx) and myeloperoxidase (MPO) activities were determined in pancreas homogenates. In VPA given group, LPO and SA levels, and ALP, TF, MPO activities significantly increased and GST, CAT, GPx activities significantly decreased compared to control group. A marked morphological damage was detected in the VPA group. Ameliorative effects of edaravone were observed in SA, TF, CAT, GPx parameters and histological examination in the VPA group. Therefore, edaravone may be effective in moderation and/or reduction of toxic effects of VPA on pancreas.
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    PublicationOpen Access
    Protective role of progesterone on lung injury induced by ischemia reperfusion of the lower limbs
    (2022-01-01) AK, ESİN; OKTAY, NİHAL ŞEHKAR; ÖZBEYLİ, DİLEK; AK, KORAY; AK E., OKTAY N. Ş., ÖZBEYLİ D., Muhan A., AK K.
    Objective: Remote lung injury is one of the most challenging issues in patients undergoing ischemia reperfusion (IR) injury of the lower limbs. We examined the role of progesterone (PG) on the remote injury of the lungs seen after IR of the lower limbs. Methods: Eighteen male Sprague Dawley rats were divided into three groups. (1) Control: rats had only two physiological saline injections intraperitoneally (i.p.) 2 h apart under general anesthesia. (2) IR: underwent 2 h tourniquet induced ischemia for both lower limbs followed by 2 h of reperfusion. Animals were injected with physiological saline (i.p.) before both ischemia and reperfusion. (3) IR + PG: rats underwent the same IR protocol as the IR group and were injected with PG (16 mg/kg, i.p.) before both ischemia and reperfusion. After reperfusion, rats were sacrificed, and lung tissues were taken out for histopathologic and biochemical analyses. Results: In IR + PG group, tissue levels of malondialdehyde and nitric oxide decreased significantly compared with the IR group (p<0.01). Similarly, glutathione level and superoxide dismutase and glutathione S-transferase activities significantly increased in the IR + PG group than in the IR group (p<0.05, p<0.01, and p<0.05, respectively). In light microscopy, reduced inflammatory cell infiltration, amelioration in alveolar structure, and mild vascular congestion in the parenchyma were seen in the IR + PG group. A significant improvement in histopathologic score was seen in the IR + PG group compared with the IR group (p<0.001). Conclusion: PG might be effective in attenuating remote injury of the lung in lower body IR via its antioxidant function