Publication: Protective role of progesterone on lung injury induced by ischemia reperfusion of the lower limbs
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Date
2022-01-01
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Abstract
Objective: Remote lung injury is one of the most challenging issues in patients undergoing
ischemia reperfusion (IR) injury of the lower limbs. We examined the role of progesterone
(PG) on the remote injury of the lungs seen after IR of the lower limbs.
Methods: Eighteen male Sprague Dawley rats were divided into three groups. (1) Control: rats had only two physiological saline injections intraperitoneally (i.p.) 2 h apart under
general anesthesia. (2) IR: underwent 2 h tourniquet induced ischemia for both lower limbs
followed by 2 h of reperfusion. Animals were injected with physiological saline (i.p.) before
both ischemia and reperfusion. (3) IR + PG: rats underwent the same IR protocol as the
IR group and were injected with PG (16 mg/kg, i.p.) before both ischemia and reperfusion.
After reperfusion, rats were sacrificed, and lung tissues were taken out for histopathologic
and biochemical analyses.
Results: In IR + PG group, tissue levels of malondialdehyde and nitric oxide decreased
significantly compared with the IR group (p<0.01). Similarly, glutathione level and superoxide dismutase and glutathione S-transferase activities significantly increased in the IR + PG
group than in the IR group (p<0.05, p<0.01, and p<0.05, respectively). In light microscopy,
reduced inflammatory cell infiltration, amelioration in alveolar structure, and mild vascular
congestion in the parenchyma were seen in the IR + PG group. A significant improvement in
histopathologic score was seen in the IR + PG group compared with the IR group (p<0.001).
Conclusion: PG might be effective in attenuating remote injury of the lung in lower body
IR via its antioxidant function
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Keywords
schemia, lower limb, lung, remote organ injury, reperfusion
Citation
AK E., OKTAY N. Ş., ÖZBEYLİ D., Muhan A., AK K., "Protective Role of Progesterone on Lung Injury Induced by Ischemia Reperfusion of the Lower Limbs", Southern Clinics of Istanbul Eurasia, cilt.33, sa.3, ss.320-325, 2022