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3-pyridinylboronic acid ameliorates rotenone-induced oxidative stress through nrf2 target genes in zebrafish embryos

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dc.contributor.authorALTURFAN, EBRU IŞIK
dc.contributor.authorMEGA TİBER, PINAR
dc.contributor.authorsÜstündağ F. D., Ünal İ., Üstündağ Ü. V., Cansız D., Beler M., Karagöz A., Kara Subaşat H., Alturfan A. A., Mega Tiber P., Emekli-Alturfan E. I.
dc.date.accessioned2023-02-14T10:33:45Z
dc.date.available2023-02-14T10:33:45Z
dc.date.issued2022-06-01
dc.description.abstractParkinson\"s disease (PD) is one of the most common forms of neurodegenerative diseases and research on potential therapeutic agents for PD continues. Rotenone is a neurotoxin that can pass the blood-brain barrier and is used to generate PD models in experimental animals. Boron is a microelement necessary for neural activity in the brain. Antioxidant, non-cytotoxic, antigenotoxic, anti-carcinogenic effects of boric acid, the salt compound of boron has been reported before. Boronic acids have been approved for treatment by FDA and are included in drug discovery studies and pyridine boronic acids are a subclass of heterocyclic boronic acids used in drug design and discovery as substituted pyridines based on crystal engineering principles. The aim of our study was to determine the effect of 3-pyridinylboronic acid in rotenone-exposed zebrafish embryos, focusing on oxidant-antioxidant parameters and gene expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) target genes gclm, gclc, hmox1a, nqo1, and PD related genes, brain-derived neurotrophic factor, dj1, and tnfa alpha. Zebrafish embryos were exposed to Rotenone (10 mu g/l); Low Dose 3-Pyridinylboronic acid (100 mu M); High Dose 3-Pyridinylboronic acid (200 mu M); Rotenone + Low Dose-3-Pyridinylboronic acid (10 mu g/l + 100 mu M); Rotenone + High Dose-3-Pyridinylboronic acid (10 mu g/l + 200 mu M) in well plates for 96 h post-fertilization (hpf). Our study showed for the first time that 3-pyridinylboronic acid, as a novel sub-class of the heterocyclic boronic acid compound, improved locomotor activities, ameliorated oxidant-antioxidant status by decreasing LPO and NO levels, and normalized the expressions of bdnf, dj1, tnf alpha and Nrf2 target genes hmox1a and nqo1 in rotenone exposed zebrafish embryos. On the other hand, it caused the deterioration of the oxidant-antioxidant balance in the control group through increased lipid peroxidation, nitric oxide levels, and decreased antioxidant enzymes. We believe that these results should be interpreted in the context of the dose-toxicity and benefit-harm relationship of the effects of 3-pyridinylboronic.
dc.identifier.citationÜstündağ F. D., Ünal İ., Üstündağ Ü. V., Cansız D., Beler M., Karagöz A., Kara Subaşat H., Alturfan A. A., Mega Tiber P., Emekli-Alturfan E. I., "3-Pyridinylboronic Acid Ameliorates Rotenone-Induced Oxidative Stress Through Nrf2 Target Genes in Zebrafish Embryos.", Neurochemical research, cilt.47, sa.1, ss.1553-1564, 2022
dc.identifier.doi10.1007/s11064-022-03548-6
dc.identifier.endpage1564
dc.identifier.issn0364-3190
dc.identifier.issue1
dc.identifier.startpage1553
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/35142995/
dc.identifier.urihttps://hdl.handle.net/11424/286230
dc.identifier.volume47
dc.language.isoeng
dc.relation.ispartofNeurochemical research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSağlık Bilimleri
dc.subjectYaşam Bilimleri
dc.subjectTemel Bilimler
dc.subjectHealth Sciences
dc.subjectLife Sciences
dc.subjectNatural Sciences
dc.subjectKlinik Tıp (MED)
dc.subjectTemel Bilimler (SCI)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectDoğa Bilimleri Genel
dc.subjectÇOK DİSİPLİNLİ BİLİMLER
dc.subjectClinical Medicine (MED)
dc.subjectNatural Sciences (SCI)
dc.subjectLife Sciences (LIFE)
dc.subjectNATURAL SCIENCES, GENERAL
dc.subjectMULTIDISCIPLINARY SCIENCES
dc.subjectMultidisciplinary
dc.subjectParkinson's disease
dc.subjectRotenone
dc.subject3-Pyridinylboronic acid
dc.subjectZebrafish embryos
dc.subjectBORIC-ACID
dc.subjectBORON-COMPOUNDS
dc.subjectDIETARY BORON
dc.subjectBRAIN
dc.subjectMETABOLITE
dc.subjectTOXICITY
dc.subjectSELENIUM
dc.subjectBORAX
dc.title3-pyridinylboronic acid ameliorates rotenone-induced oxidative stress through nrf2 target genes in zebrafish embryos
dc.typearticle
dspace.entity.typePublication
local.avesis.id3ba9cdc4-734b-4177-8b7d-af542934cd3d
local.indexed.atWOS
local.indexed.atPUBMED
local.indexed.atSCOPUS
relation.isAuthorOfPublicationb1be167b-6305-44c3-9a1b-f4437290d8d3
relation.isAuthorOfPublication68991f0d-2fc8-45a5-9ebc-1d3c95afe742
relation.isAuthorOfPublication.latestForDiscoveryb1be167b-6305-44c3-9a1b-f4437290d8d3

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