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Quercetin treatment against ischemia/reperfusion injury in rat corpus cavernosum tissue: a role on apoptosis and oxidative stress

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dc.contributor.authorŞENER, TARIK EMRE
dc.contributor.authorsCevik, O.; Cadirci, S.; Sener, T. E.; Tinay, I.; Akbal, C.; Tavukcu, H. H.; Cetinel, S.; Kiran, D.; Sener, G.
dc.date.accessioned2022-03-12T18:10:29Z
dc.date.available2022-03-12T18:10:29Z
dc.date.issued2013
dc.description.abstractReactive oxygen metabolites play an important role in the ischemia/reperfusion (I/R)-induced tissue injury. This study was designed to investigate the possible protective effects of quercetin against I/R injury of the rat corpus cavernosum tissue. To induce I/R injury, abdominal aorta was clamped for 30 min and reperfused for 60 min. Quercetin (20 mg/kg) or vehicle was given before ischemia and just after reperfusion in the I/R group and in the sham-operated control group in which clamping was not performed. After decapitation, corpus cavernosum tissues were removed and either placed in organ baths or stored for evaluating biochemical parameters. Oxidative injury was examined by measuring lucigenin chemiluminescence (CL), nitric oxide (NO), malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD) and myeloperoxidase (MPO) activities and caspase-3 protein levels. In the I/R group, contractile responses to phenylephrine and relaxation responses to carbachol were impaired significantly compared with those in the control groups, while quercetin treatment in I/R group reversed both of the responses. On the other hand, increase in lucigenin CL, NO, MDA levels and MPO and caspase-3 activities and decrease in GSH levels and SOD activity in the cavernosal tissues of the I/R group were also significantly reversed by quercetin treatment. Furthermore, observed distorted morphology with ruptured endothelial cells and vacuolization in the cytoplasm of cavernosal tissues of I/R no longer persisted in the quercetin-treated I/R group. Thus, our results suggested that treatment with quercetin may have some benefits in controlling I/R-induced tissue injury through its anti-inflammatory, anti-apoptotic, and antioxidant effects.
dc.identifier.doi10.3109/10715762.2013.814912
dc.identifier.eissn1029-2470
dc.identifier.issn1071-5762
dc.identifier.pubmed23758074
dc.identifier.urihttps://hdl.handle.net/11424/231384
dc.identifier.wosWOS:000323107900003
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS LTD
dc.relation.ispartofFREE RADICAL RESEARCH
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectquercetin
dc.subjectischemia/reperfusion
dc.subjectantioxidant
dc.subjectapoptosis
dc.subjectinflammation
dc.subjectcorpus cavernosum
dc.subjectERECTILE DYSFUNCTION
dc.subjectPENILE ARTERIES
dc.subjectPROTECTIVE ROLE
dc.subjectNITRIC-OXIDE
dc.subjectISCHEMIA
dc.subjectANTIOXIDANT
dc.subjectMELATONIN
dc.subjectMYELOPEROXIDASE
dc.subjectINFLAMMATION
dc.subjectMECHANISMS
dc.titleQuercetin treatment against ischemia/reperfusion injury in rat corpus cavernosum tissue: a role on apoptosis and oxidative stress
dc.typearticle
dspace.entity.typePublication
local.avesis.ida77c2926-a273-4320-96f2-43910badc096
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages9
oaire.citation.endPage691
oaire.citation.issue9
oaire.citation.startPage683
oaire.citation.titleFREE RADICAL RESEARCH
oaire.citation.volume47
relation.isAuthorOfPublication5ff4af68-b410-4de6-be8b-a4a80167107e
relation.isAuthorOfPublication.latestForDiscovery5ff4af68-b410-4de6-be8b-a4a80167107e

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