Publication:
The role of the substantia nigra pars reticulata in kindling resistance in rats with genetic absence epilepsy

Simple item page
dc.contributor.authorONAT, FİLİZ
dc.contributor.authorsAkman, Ozlem; Gulcebi, Medine I.; Carcak, Nihan; Ozatman, Sema Ketenci; Eryigit, Tugba; Moshe, Solomon L.; Galanopoulou, Aristea S.; Onat, Filiz Yilmaz
dc.date.accessioned2022-03-14T11:10:44Z
dc.date.available2022-03-14T11:10:44Z
dc.date.issued2015-11
dc.description.abstractObjectiveGenetic Absence Epilepsy Rats from Strasbourg (GAERS) show a resistance to secondary generalization of focal limbic seizures evoked by kindling. The substantia nigra pars reticulata (SNR) is involved in the propagation and modulation of seizures in kindling. We first examined the role of the SNRanterior and SNRposterior subregions in the resistance to the development of kindling in GAERS. Subsequently, to determine whether kindling resistance relates to differential sensitivity of -aminobutyric acid -aminobutyric acid (GABA)ergic or dopaminergic SNR neurons to kindling, we studied the effects of kindling-inducing stimulations on parvalbumin (PRV; GABAergic neuron marker) or tyrosine hydroxylase (TH; dopaminergic neuron marker) immunoreactivity (ir), respectively, in GAERS and in nonepileptic control (NEC) Wistar rats that lack kindling resistance. MethodsAdult male GAERS were implanted with a stimulation electrode in the amygdala, and bilateral injection cannulas for lidocaine or saline injection (30 min before each kindling stimulation until the animals reached three stage 5 seizures or the 22 stimulations) into the SNRanterior or SNRposterior. In another experiment, PRV-ir in SNRanterior and SNRposterior and TH-ir in SNRposterior only were densitometrically compared in GAERS-SHAM, NEC-SHAM GAERS-STIM, and NEC-STIM animals (6 kindling stimulations). ResultsBilateral SNRposterior infusions of lidocaine eliminated the kindling resistance and resulted in stage 5 generalized motor seizures in all kindled rats. Bilateral lidocaine infusions in the SNRanterior failed to alter the kindling resistance in GAERS. PRV-ir in the SNRposterior was unaltered in GAERS-STIM but increased in NEC-STIM group. Cellular TH-ir in the SNRposterior significantly increased by kindling stimulations in both NEC-STIM and GAERS-STIM groups. SignificanceThe kindling resistance in GAERS is mediated by the SNRposterior in a lidocaine-sensitive manner. The insensitivity to kindling stimulation of PRV-ir in SNRposterior of GAERS but not NEC rats, implicate GABAergic SNRposterior neurons in kindling resistance. In contrast, the observed stimulation-specific increase in TH-ir in the SNRposterior is unrelated to kindling resistance.
dc.identifier.doi10.1111/epi.13204
dc.identifier.eissn1528-1167
dc.identifier.issn0013-9580
dc.identifier.pubmed26471261
dc.identifier.urihttps://hdl.handle.net/11424/246001
dc.identifier.wosWOS:000363884100025
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofEPILEPSIA
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGAERS
dc.subjectKindling resistance
dc.subjectSubstantia nigra pars reticulata
dc.subjectTyrosine hydroxylase
dc.subjectParvalbumin
dc.subjectDEVELOPMENTAL REGULATION
dc.subjectTYROSINE-HYDROXYLASE
dc.subjectGABA(A) RECEPTOR
dc.subjectWAVE DISCHARGES
dc.subjectBASAL GANGLIA
dc.subjectSEIZURES
dc.subjectNEURONS
dc.subjectMICROINFUSIONS
dc.subjectORGANIZATION
dc.subjectSTIMULATION
dc.titleThe role of the substantia nigra pars reticulata in kindling resistance in rats with genetic absence epilepsy
dc.typearticle
dspace.entity.typePublication
local.avesis.idfcb25fa5-461d-48b5-ad0a-af412f430af0
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages10
oaire.citation.endPage1802
oaire.citation.issue11
oaire.citation.startPage1793
oaire.citation.titleEPILEPSIA
oaire.citation.volume56
relation.isAuthorOfPublicationc359dea3-046f-4397-90d5-62e4bfc31869
relation.isAuthorOfPublication.latestForDiscoveryc359dea3-046f-4397-90d5-62e4bfc31869

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Akman et al. - 2015 - The role of the substantia nigra pars reticulata i.pdf
Size:
534.64 KB
Format:
Adobe Portable Document Format
Download

Collections

Research Outputs