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Apocynin attenuates testicular ischemia-reperfusion injury in rats

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dc.contributor.authorŞİMŞEK, FERRUH
dc.contributor.authorsSener, T. Emre; Yuksel, Meral; Ozyilmaz-Yay, Nagehan; Ercan, Feriha; Akbal, Cem; Simsek, Ferruh; Sener, Goksel
dc.date.accessioned2022-03-13T12:47:23Z
dc.date.available2022-03-13T12:47:23Z
dc.date.issued2015
dc.description.abstractObjective: This study was designed to examine the possible protective effect of apocynin, a NADPH oxidase inhibitor, against torsion/detorsion (T/D) induced ischemia/reperfusion (I/R) injury in testis. Methods: Male Wistar albino rats were divided into sham-operated control, and either vehicle, apocynin 20 mg/kg-or apocynin 50 mg/kg-treated T/D groups. In order to induce I/R injury, left testis was rotated 720 degrees clockwise for 4 hours (torsion) and then allowed reperfusion (detorsion) for 4 hours. Left orchiectomy was done for the measurement of tissue malondialdehyde (MDA), glutathione (GSH) levels, myeloperoxidase (MPO) activity, and luminol, lucigenin, nitric oxide (NO) and peroxynitrite chemiluminescences (CL). Testicular morphology was examined by light microscopy. Results: I/R caused significant increases in tissue luminol, lucigenin, nitric oxide and peroxynitrite CL demonstrating increased reactive oxygen and nitrogen metabolites. As a result of increased oxidative stress tissue MPO activity, MDA levels were increased and antioxidant GSH was decreased. On the other hand, apocynin treatment reversed all these biochemical indices, as well as histopathological alterations that were induced by I/R. According to data, although lower dose of apocynin tended to reverse the biochemical parameters, high dose of apocynin provides better protection since values were closer to the control levels. Conclusion: Findings of the present study suggest that NADPH oxidase inhibitor apocynin by inhibiting free radical generation and increasing antioxidant defense exerts protective effects on testicular tissues against I/R. The protection with apocynin was more pronounced with high dose. (C) 2015 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.jpedsurg.2014.11.033
dc.identifier.eissn1531-5037
dc.identifier.issn0022-3468
dc.identifier.pubmed25783298
dc.identifier.urihttps://hdl.handle.net/11424/238068
dc.identifier.wosWOS:000360121000028
dc.language.isoeng
dc.publisherW B SAUNDERS CO-ELSEVIER INC
dc.relation.ispartofJOURNAL OF PEDIATRIC SURGERY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSpermatic cord torsion
dc.subjectTestis
dc.subjectReperfusion injury
dc.subjectApocynin
dc.subjectOXIDATIVE STRESS
dc.subjectNADPH OXIDASE
dc.subjectFREE-RADICALS
dc.subjectDAMAGE
dc.subjectGLUTATHIONE
dc.subjectMECHANISMS
dc.subjectHYPERTENSION
dc.subjectINFLAMMATION
dc.subjectSUPEROXIDE
dc.subjectEXPRESSION
dc.titleApocynin attenuates testicular ischemia-reperfusion injury in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id71bbcec9-4093-448a-b597-477e56e5c8fd
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage1387
oaire.citation.issue8
oaire.citation.startPage1382
oaire.citation.titleJOURNAL OF PEDIATRIC SURGERY
oaire.citation.volume50
relation.isAuthorOfPublicationb91c9805-ef29-4cda-82ba-4b4da6191e26
relation.isAuthorOfPublication.latestForDiscoveryb91c9805-ef29-4cda-82ba-4b4da6191e26

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