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Mesna (2-mercaptoethane sulfonate) prevents ischemia/reperfusion induced renal oxidative damage in rats

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dc.contributor.authorŞENER, GÖKSEL
dc.contributor.authorsKabasakal, L; Sehirli, AO; Cetinel, S; Cikler, E; Gedik, N; Sener, G
dc.date.accessioned2022-03-12T17:16:44Z
dc.date.available2022-03-12T17:16:44Z
dc.date.issued2004
dc.description.abstractReoxygenation of the ischemic tissue promotes the generation of various reactive oxygen metabolites (ROM) which are known to have deleterious effects on various cellular functions. This study was designed to determine the possible protective effect of mesna (2-Mercaptoethane Sulfonate) on renal ischemia/reperfusion (I/R) injury. Wistar albino rats were unilaterally nephrectomized, and 15 days later they were subjected to 45 min of renal pedicle occlusion followed by 6 h of reperfusion. Mesna (MESNA, 150 mg/kg, i.p.; an effective dose against I/R injury) or vehicle was administered twice, 15 min prior to ischemia and immediately before the reperfusion period. At the end of the reperfusion period, rats were killed by decapitation. Kidney samples were taken for histological examination or determination of the free radicals, renal malondialdehyde (MDA) and glutathione (GSH) levels, and myeloperoxidase (MPO) activity. Renal tissue collagen content, as a fibrosis marker was also determined. Creatinine and urea concentrations in blood were measured for the evaluation of renal function. The results demonstrated that renal I/R caused nephrotoxicity, as evidenced by increases in blood urea and creatinine levels, which was reversed by MESNA treatment. Increased free radical levels, as assessed by nitroblue-tetrazolium test were reduced with MESNA. Moreover, the decrease in GSH and increases in MDA levels, and MPO activity induced by I/R indicated that renal injury involves free radical formation. Treatment of rats with MESNA restored the reduced GSH levels while it decreased MDA levels as well as MPO activity. Increased collagen contents of the kidney tissues by I/R were reversed back to the control levels by MESNA treatment. Since MESNA administration reversed these oxidant responses, improved renal function and microscopic damage, it seems likely that MESNA protects kidney tissue against I/R induced oxidative damage. (C) 2004 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.lfs.2004.04.029
dc.identifier.eissn1879-0631
dc.identifier.issn0024-3205
dc.identifier.pubmed15350830
dc.identifier.urihttps://hdl.handle.net/11424/227673
dc.identifier.wosWOS:000223883300007
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofLIFE SCIENCES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectischemia/reperfusion
dc.subjectmesna
dc.subjectlipid peroxidation
dc.subjectglutathione
dc.subjectmyeloperoxidase
dc.subjectISCHEMIA-REPERFUSION
dc.subjectTISSUE-INJURY
dc.subjectFREE-RADICALS
dc.subjectMYELOPEROXIDASE ACTIVITY
dc.subjectLIPID-PEROXIDATION
dc.subjectN-ACETYLCYSTEINE
dc.subjectKIDNEY
dc.subjectANTIOXIDANT
dc.subjectDYSFUNCTION
dc.subjectMELATONIN
dc.titleMesna (2-mercaptoethane sulfonate) prevents ischemia/reperfusion induced renal oxidative damage in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.ida128e7e9-d42f-4376-a007-4ebe5c3828e5
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages12
oaire.citation.endPage2340
oaire.citation.issue19
oaire.citation.startPage2329
oaire.citation.titleLIFE SCIENCES
oaire.citation.volume75
relation.isAuthorOfPublication2fd85a89-446e-49e1-98c9-d20b11a1a2b5
relation.isAuthorOfPublication.latestForDiscovery2fd85a89-446e-49e1-98c9-d20b11a1a2b5

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