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Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress

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dc.contributor.authorTOKSOY ÖNER, EBRU
dc.contributor.authorsQueiroz, Eveline A. I. F.; Fortes, Zuleica B.; da Cunha, Mario A. A.; Sarilmiser, Hande Kazak; Dekker, Aneli M. Barbosa; Oner, Ebru Toksoy; Dekker, Robert F. H.; Khaper, Neelam
dc.date.accessioned2022-03-12T22:24:12Z
dc.date.available2022-03-12T22:24:12Z
dc.date.issued2017
dc.description.abstractExopolysaccharides are high-valued bio-products produced by various microbial species and have been described to possess biological response modifying activities. These bio-products have been effective as therapeutic agents in various human disease conditions. The objective of this study was to examine the effects of levan (a (2 -> 6)-beta-D-fructan) produced on sucrose by the halophilic bacterium, Halomonas smyrnensis AAD6(T), in human breast cancer MCF-7 cells. MCF-7 cells were exposed to levan for 24 and 48 h. The antiproliferative activity was analyzed by the MTF assay. Oxidative stress was measured by the CM-H(2)DCFDA assay, and cell apoptosis was analyzed by the caspase-3/7 assay. Cell cycle was analyzed by flow cytometry and gene expression was determined by RT-PCR. Levan showed a time- and concentration-dependent antiproliferative activity, and this effect was associated with an increase in cell apoptosis and oxidative stress. In addition, levan increased the gene expression of p53 and p27. Here we demonstrated that levan exhibited an antiproliferative effect that was mediated by an increase in apoptosis and oxidative stress. (C) 2017 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.ijbiomac.2017.04.035
dc.identifier.eissn1879-0003
dc.identifier.issn0141-8130
dc.identifier.pubmed28412340
dc.identifier.urihttps://hdl.handle.net/11424/234702
dc.identifier.wosWOS:000406984300062
dc.language.isoeng
dc.publisherELSEVIER
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject(2 -> 6)-beta-D-Fructan
dc.subjectApoptosis
dc.subjectp53
dc.subjectp27
dc.subjectOxidative stress
dc.subjectFORKHEAD TRANSCRIPTION FACTOR
dc.subjectANTITUMOR-ACTIVITY
dc.subjectMOLECULAR-WEIGHT
dc.subjectBETA-GLUCANS
dc.subjectANTIOXIDANT
dc.subjectPOLYSACCHARIDES
dc.subjectFERMENTATION
dc.subjectINHIBITION
dc.subjectINDUCTION
dc.subjectFAMILY
dc.titleLevan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress
dc.typearticle
dspace.entity.typePublication
local.avesis.id8fbfb7dd-0132-423c-aa5b-f9c2375288fb
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
local.journal.quartileQ1
oaire.citation.endPage570
oaire.citation.startPage565
oaire.citation.titleINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
oaire.citation.volume102
relation.isAuthorOfPublication6118e9e4-a58e-429b-bbec-4f73a3089a2b
relation.isAuthorOfPublication.latestForDiscovery6118e9e4-a58e-429b-bbec-4f73a3089a2b

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