Publication:
Chard (Beta vulgaris L. var. cicla) extract ameliorates hyperglycemia by increasing GLUT2 through Akt2 and antioxidant defense in the liver of rats

Simple item page
dc.contributor.authorŞENER, GÖKSEL
dc.contributor.authorsGezginci-Oktayoglu, Selda; Sacan, Ozlem; Bolkent, Sehnaz; Ipci, Yesim; Kabasakal, Levent; Sener, Goksel; Yanardag, Refiye
dc.date.accessioned2022-03-13T12:45:15Z
dc.date.available2022-03-13T12:45:15Z
dc.date.issued2014
dc.description.abstractChard is a plant used as an alternative hypoglycemic agent by diabetic people in Turkey. The aim of this study was to examine the molecular mechanism of hypoglycemic effects of chard extract. Male Sprague-Dawley rats (6-7 months old) were divided into five groups for this investigation: (1) control, (2) hyperglycemic, (3) hyperglycemic+chard, (4) hyperglycemic + insulin, (5) hyperglycemic + chard + insulin. Fourteen days after animals were rendered hyperglycemic by intraperitoneal injection of 60 mg/kg streptozotocin, the chard water extract (2 g/kg/day) or/and insulin (6 U/kg/day) was administered for 45 days. Hypoglycemic effect of chard extract was demonstrated by a significant reduction in the fasting blood glucose and increased glycogen levels in liver of chard extract-treated hyperglycemic rats. Moreover, activity of adenosine deaminase, which is suggested as an important enzyme for modulating the bioactivity of insulin, was decreased by chard treatment. Immunostaining analysis showed increased nuclear translocation of Akt2 and synthesis of GLUT2 in the hepatocytes of chard or/and insulin-treated hyperglycemic rats. The oxidative stress was decreased and antioxidant defense was increased by chard extract or/and insulin treatment to hyperglycemic rats according to the decreased malondialdehyde formation, the activities of catalase, superoxide dismutase, myeloperoxidase and increased glutathione levels. These findings suggest that chard extract might improve glucose response by increasing GLUT2 through Akt2 and antioxidant defense in the liver. (C) 2013 Elsevier GmbH. All rights reserved.
dc.identifier.doi10.1016/j.acthis.2013.04.016
dc.identifier.eissn1618-0372
dc.identifier.issn0065-1281
dc.identifier.pubmed23746671
dc.identifier.urihttps://hdl.handle.net/11424/237747
dc.identifier.wosWOS:000331671300006
dc.language.isoeng
dc.publisherELSEVIER GMBH
dc.relation.ispartofACTA HISTOCHEMICA
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAkt2
dc.subjectChard extract
dc.subjectHyperglycemia
dc.subjectGLUT2
dc.subjectLiver
dc.subjectOxidative stress
dc.subjectINSULIN-RESISTANCE
dc.subjectLIPID-PEROXIDATION
dc.subjectPOTENTIAL ROLE
dc.subjectKINASE
dc.subjectSTREPTOZOTOCIN
dc.subjectDNA
dc.subjectACTIVATION
dc.subjectTOXICITY
dc.subjectVESICLES
dc.subjectGLYCOGEN
dc.titleChard (Beta vulgaris L. var. cicla) extract ameliorates hyperglycemia by increasing GLUT2 through Akt2 and antioxidant defense in the liver of rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id3e8c6176-dae2-4fa6-8211-e028965fe83c
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages8
oaire.citation.endPage39
oaire.citation.issue1
oaire.citation.startPage32
oaire.citation.titleACTA HISTOCHEMICA
oaire.citation.volume116
relation.isAuthorOfPublication2fd85a89-446e-49e1-98c9-d20b11a1a2b5
relation.isAuthorOfPublication.latestForDiscovery2fd85a89-446e-49e1-98c9-d20b11a1a2b5

Files

Collections

Research Outputs