Publication:
Alkaline phosphatase activity is unregulated in regenerating human periodontal cells

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dc.contributor.authorKURU, LEYLA
dc.contributor.authorsKuru, L; Griffiths, GS; Petrie, A; Olsen, I
dc.date.accessioned2022-03-12T17:01:34Z
dc.date.available2022-03-12T17:01:34Z
dc.date.issued1999
dc.description.abstractThe activity of alkaline phosphatase (ALP) is considered to indicate the presence of osteoblast cells and the formation of new bone. In the present study this enzyme was investigated in cells obtained from retrieved polytetrafluoroethylene membranes (M cells) of periodontal disease patients treated by guided tissue regeneration (GTR) and from the regenerated tissue underlying the membrane (RT cells). Normal periodontal ligament (PL) and gingival cells were also grown from the corresponding healthy tissues of human subjects. ALP activity was measured colourimetrically, using paranitrophenyl phosphate as the substrate, after 4 and 7 d of culture in the absence and presence of dexamethasone (DEX), a synthetic glucocorticoid which induces osteoblast differentiation. The results showed that basal levels of ALP activity were expressed by all the cells and that DEX upregulated ALP levels in the M, RT and PL cells but not in the gingival cells. Moreover, both the basal and DEX-induced ALP activities were statistically significantly higher in the RT cells than in any of the other cells. Our results suggest that both the GTR-associated and normal PL cells express osteoblast-like characteristics and, furthermore, that the RT cultures in particular contain a high proportion of osteoprogenitor cells.
dc.identifier.doi10.1111/j.1600-0765.1999.tb02231.x
dc.identifier.eissn1600-0765
dc.identifier.issn0022-3484
dc.identifier.pubmed10207841
dc.identifier.urihttps://hdl.handle.net/11424/227391
dc.identifier.wosWOS:000079333700008
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofJOURNAL OF PERIODONTAL RESEARCH
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalkaline phosphatase activity
dc.subjectGTR
dc.subjectcell culture
dc.subjectdexamethasone
dc.subjectGUIDED TISSUE REGENERATION
dc.subjectEXPANDED POLYTETRAFLUOROETHYLENE MEMBRANES
dc.subjectHUMAN INFRABONY DEFECTS
dc.subjectIMMUNOHISTOCHEMICAL ANALYSIS
dc.subjectATTACHMENT FORMATION
dc.subjectLIGAMENT
dc.subjectDIFFERENTIATION
dc.subjectFIBROBLASTS
dc.subjectADHERENT
dc.subjectMARROW
dc.titleAlkaline phosphatase activity is unregulated in regenerating human periodontal cells
dc.typearticle
dspace.entity.typePublication
local.avesis.id957f1751-40b5-4bfe-88d1-7a3245672a3d
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages5
oaire.citation.endPage127
oaire.citation.issue2
oaire.citation.startPage123
oaire.citation.titleJOURNAL OF PERIODONTAL RESEARCH
oaire.citation.volume34
relation.isAuthorOfPublicationcc49fe59-fb00-4a6a-a32c-60d68c5f3993
relation.isAuthorOfPublication.latestForDiscoverycc49fe59-fb00-4a6a-a32c-60d68c5f3993

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