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Pro-inflammatory cytokine and caspase-1 responses to pattern recognition receptor activation of neutrophils and dendritic cells in Behcet's disease

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dc.contributor.authorMUMCU, GONCA
dc.contributor.authorsTure-Ozdemir, Filiz; Tulunay, Aysin; Elbasi, Mehmet Onur; Tatli, Imren; Maurer, Anne-Marie; Mumcu, Gonca; Direskeneli, Haner; Eksioglu-Demiralp, Emel
dc.date.accessioned2022-03-14T10:54:30Z
dc.date.available2022-03-14T10:54:30Z
dc.date.issued2013-05-01
dc.description.abstractObjective. Activated innate immunity is implicated in the pathogenesis of Behcet's disease (BD). To clarify the mechanisms of innate immune responses, we investigated inflammasome activation in dendritic cells (DCs) and neutrophils, following stimulation with two different pattern recognition receptors (PRRs) RIG-1-like (RLR) and NOD-like (NLR) in patients with BD. Methods. Sixteen active BD patients with mucocutaneous lesions and 17 healthy controls (HCs) were included in this study. DCs were generated from monocytes. DCs and isolated neutrophils were activated by RLR and NLR ligands. Caspase-1 activation and expression of p38 and RIP2 were determined by flow cytometry. Levels of IL-1 beta, IL-6, TNF-alpha, IFN-alpha and IL-18 in culture supernatants were measured by ELISA. Results. Activation of caspase-1 following intracellular PRR stimulation was found to be of similar levels in DCs and neutrophils of BD patients compared with HCs. However, activation of DCs from BD patients to NOD2 stimulus measured by the expression of RIP2 and p38 as well as IL-18 levels was found to be slightly defective (P < 0.05). In neutrophil cultures, IL-6 levels were lower in response to all stimuli in patients with BD compared with HCs (P < 0.01). Conclusion. Inflammasome formation following stimulation with NOD1/NOD2 and RIG measured by caspase-1 activation, cytokine levels and expression of RIP2 and p38 seems to be functionally normal in DCs and neutrophils of BD patients, although slightly defective responses in some pathways and cytokine levels were observed. These results may suggest that caspase-1-independent pathways such as toll-like receptors may be more prominent in BD pathogenesis.
dc.identifier.doi10.1093/rheumatology/kes399
dc.identifier.issn1462-0324
dc.identifier.pubmed23325038
dc.identifier.urihttps://hdl.handle.net/11424/245424
dc.identifier.wosWOS:000318114400009
dc.language.isoeng
dc.publisherOXFORD UNIV PRESS
dc.relation.ispartofRHEUMATOLOGY
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBehcet's disease
dc.subjectdendritic cells
dc.subjectinflammasome
dc.subjectneutrophils
dc.subjectpattern recognition receptors
dc.subjectINFECTIOUS ETIOLOGY
dc.subjectINNATE IMMUNITY
dc.subjectMANIFESTATIONS
dc.subjectPATHOGENESIS
dc.subjectASSOCIATION
dc.subjectEXPRESSION
dc.subjectNOD2
dc.titlePro-inflammatory cytokine and caspase-1 responses to pattern recognition receptor activation of neutrophils and dendritic cells in Behcet's disease
dc.typearticle
dspace.entity.typePublication
local.avesis.idc481d54b-0f55-424a-8c61-47b59f77ad92
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages6
oaire.citation.endPage805
oaire.citation.issue5
oaire.citation.startPage800
oaire.citation.titleRHEUMATOLOGY
oaire.citation.volume52
relation.isAuthorOfPublication15fb5c8c-4e72-485b-bf3d-c6e191e3f5aa
relation.isAuthorOfPublication.latestForDiscovery15fb5c8c-4e72-485b-bf3d-c6e191e3f5aa

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