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An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat

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dc.contributor.authorAydın, Yıldız
dc.contributor.authorAltinkut Uncuoǧlu, Ahu
dc.contributor.departmentDepartment of Biology, Marmara Universityen_US
dc.date.accessioned2016-04-15T18:08:55Z
dc.date.available2016-04-15T18:08:55Z
dc.date.issued2014
dc.description.abstractEST-SSR markers, derived from the A and B genomes of wheat were used to identify molecular markers associated with yellow rust resistance. For this purpose, bulk segregant analysis was performed using 114 EST-SSR primer pairs. They were screened on the parent genotypes and resistant/susceptible DNA pools from the cross between Izgi2001 (resistant male parent) × ES14 (susceptible female parent) at the seedling and adult plant stage. An EST-SSR marker, bu099658, generated the 206 bp DNA fragment that was present in the resistant parent and resistant bulk, but it was not present in the susceptible parent and the susceptible bulk. To investigate its association with Yr genes, 20 individuals of NILs were also amplified with BU099658 and the 206 bp marker fragment was obtained only in Yr1/6 × Avocet S. Additionally, bu099658 was screened on 65 genotypes which possessed different Yr genes/gene combination(s) and Yr1. The results indicate a close linkage of bu099658 with the Yr1 gene.en_US
dc.identifier.endpage18en_US
dc.identifier.issn12121975
dc.identifier.issue1en_US
dc.identifier.startpage11en_US
dc.identifier.urihttp://tinyurl.com/z5jutm2
dc.identifier.urihttp://hdl.handle.net/11424/4432
dc.identifier.volume11en_US
dc.language.isoengen_US
dc.relation.journalCzech Journal of Genetics and Plant Breedingen_US
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.subjectBulk segregant analysis (BSA); Marker-assisted selection (MAS); Puccinia striiformis f.sp.tritici; Triticum aestivum L.; Yr1en_US
dc.titleAn EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheaten_US
dc.typearticleen_US
dspace.entity.typePublication
local.import.packageSS40

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