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Amelioration of methotrexate-induced enteritis by melatonin in rats

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dc.contributor.authorŞENER, GÖKSEL
dc.contributor.authorsJahovic, N; Sener, G; Cevik, H; Ersoy, Y; Arbak, S; Yegen, BC
dc.date.accessioned2022-03-12T17:16:26Z
dc.date.available2022-03-12T17:16:26Z
dc.date.issued2004
dc.description.abstractThe anti-tumour drug methotrexate (MTX) induces intestinal mucosa injury resulting in malabsorption and diarrhoea. The purpose of this study wag to investigate whether exogenous melatonin could protect the gut from MTX-induced damage in rats. A single dose of MTX (20 mg kg(-1), i.p.) was followed by i.p. saline or melatonin injections (10 mg kg(-1), MTX + Mel) for the next 5 days. On the fifth day, intestinal transit was assessed using charcoal propagation. Rats were decapitated and small intestinal segments were fixed for light (LM) and scanning electron microscope (SEM) examinations. Other intestinal segments were stored to measure glutathione (GSH) and malondialdehyde (MDA) levels, myeloperoxidase (MPO) and ATPase activity. MTX led to loss of more than 10% of the initial body weight (p < 0.01). Conversely, weight loss was markedly less in the melatonin-treated MTX group (p < 0.05). Bowel motility was increased in MTX-treated rats, while the transit index in the MTX-Mel group was not different from the control group. MTX caused decreases in GSH levels and ATPase activity. with increases in MDA levels and MPO activity. These changes were reversed in MTX-Mel-treated rats (p < 0.05-p < 0.001). LM and SEM in the MTX group revealed desquamation of surface epithelium and glandular degeneration, while the epithelium was slightly damaged in the MTX-Mel group. In conclusion, the present study demonstrates that melatonin is capable of reversing MTX-induced intestinal dysfunctions, indicating that it may be beneficial in ameliorating the symptoms of chemotherapy-induced enteritis. Copyright (C) 2004 John Wiley Sons, Ltd.
dc.identifier.doi10.1002/cbf.1071
dc.identifier.eissn1099-0844
dc.identifier.issn0263-6484
dc.identifier.pubmed15124182
dc.identifier.urihttps://hdl.handle.net/11424/227576
dc.identifier.wosWOS:000221501300007
dc.language.isoeng
dc.publisherWILEY
dc.relation.ispartofCELL BIOCHEMISTRY AND FUNCTION
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectmethotrexate
dc.subjectmelatonin
dc.subjectenteritis
dc.subjectmyeloperoxidase activity
dc.subjectNa+-K+-ATPase activity
dc.subjectSMALL-INTESTINE
dc.subjectOXIDATIVE STRESS
dc.subjectDAMAGE
dc.subjectACID
dc.subjectCELL
dc.subjectDEHYDROGENASES
dc.subjectLOCALIZATION
dc.subjectTRANSPORT
dc.titleAmelioration of methotrexate-induced enteritis by melatonin in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id0ff5d79d-7cd4-4bbd-a054-5cbeda6739e6
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages10
oaire.citation.endPage178
oaire.citation.issue3
oaire.citation.startPage169
oaire.citation.titleCELL BIOCHEMISTRY AND FUNCTION
oaire.citation.volume22
relation.isAuthorOfPublication2fd85a89-446e-49e1-98c9-d20b11a1a2b5
relation.isAuthorOfPublication.latestForDiscovery2fd85a89-446e-49e1-98c9-d20b11a1a2b5

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