Publication:
Protective effect of aqueous garlic extract against renal ischemia/reperfusion injury in rats

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dc.contributor.authorŞENER, GÖKSEL
dc.contributor.authorsKabasakal, L; Sehirli, O; Cetinel, S; Cikler, E; Gedik, N; Sener, G
dc.date.accessioned2022-03-12T17:20:12Z
dc.date.available2022-03-12T17:20:12Z
dc.date.issued2005
dc.description.abstractOxygen free radicals are important components involved in pathophysiological tissue alteration observed during ischemia/reperfusion (I/R). This study was designed to determine the possible protective effect of aqueous garlic extract (AGE) on renal I/R injury. Wistar albino rats were unilaterally nephrectomized and subjected to 45 minutes of renal pedicle occlusion followed by 6 hours of reperfusion. AGE (I mL/kg, i.p., corresponding to 500 mg/kg) or vehicle was administered twice: 15 minutes prior to ischemia and immediately before the reperfusion period. At the end of the reperfusion period, rats were killed by decapitation. Kidney samples were taken for histological examination or determination of levels of free radicals; renal malondialdehyde (MDA), an end product of lipid peroxidation; glutathione (GSH), a key antioxidant; and myeloperoxidase (MPO) activity, an index of tissue neutrophil infiltration. Renal tissue collagen content, as a fibrosis marker, was also determined. Creatinme and urea concentrations in blood were measured for the evaluation of renal function. The results revealed that I/R-induced nephrotoxicity, as evidenced by increases in blood urea and creatinine levels, was reversed by AGE treatment. The levels of free radicals, as assessed by the nitro blue tetrazolium test, were increased. Moreover, the decrease in GSH levels and the increases in MDA levels and MPO activity induced by I/R indicated that renal injury involves free radical formation. Treatment of rats with AGE (1 mL/kg) restored the reduced GSH levels, while it decreased free levels of radicals and MDA as well as MPO activity. Collagen contents of the kidney tissues increased by I/R were reversed back to the control levels with AGE. Since AGE administration reversed these oxidant responses and improved renal function and damage at the microscopic level, it seems likely that AGE protects kidney tissue against I/R-induced oxidative damage.
dc.identifier.doi10.1089/jmf.2005.8.319
dc.identifier.eissn1557-7600
dc.identifier.issn1096-620X
dc.identifier.pubmed16176141
dc.identifier.urihttps://hdl.handle.net/11424/228208
dc.identifier.wosWOS:000232239100006
dc.language.isoeng
dc.publisherMARY ANN LIEBERT, INC
dc.relation.ispartofJOURNAL OF MEDICINAL FOOD
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectgarlic
dc.subjectglutathione
dc.subjectischemia/reperfusion
dc.subjectlipid peroxidation
dc.subjectmyeloperoxidase
dc.subjectISCHEMIA-REPERFUSION INJURY
dc.subjectVASCULAR ENDOTHELIAL-CELLS
dc.subjectLIPID-PEROXIDATION
dc.subjectOXIDATIVE STRESS
dc.subjectTISSUE-INJURY
dc.subjectFREE-RADICALS
dc.subjectMYELOPEROXIDASE ACTIVITY
dc.subjectNEUTROPHIL INFILTRATION
dc.subjectRISK-FACTORS
dc.subjectGLUTATHIONE
dc.titleProtective effect of aqueous garlic extract against renal ischemia/reperfusion injury in rats
dc.typearticle
dspace.entity.typePublication
local.avesis.ida722bd15-2b3c-4116-9278-d1120be0df98
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages8
oaire.citation.endPage326
oaire.citation.issue3
oaire.citation.startPage319
oaire.citation.titleJOURNAL OF MEDICINAL FOOD
oaire.citation.volume8
relation.isAuthorOfPublication2fd85a89-446e-49e1-98c9-d20b11a1a2b5
relation.isAuthorOfPublication.latestForDiscovery2fd85a89-446e-49e1-98c9-d20b11a1a2b5

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