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The effect of betulinic acid on TNBS-induced experimental colitis [Betulinik asitin TNBS ile oluşturulan deneysel kolit üzerine etkileri]

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dc.contributor.authorYEGEN, BERRAK
dc.contributor.authorsŞener T.E., Kardaş R.C., Şehirli A.Ö., Ekşioǧlu-Demiral E., Yüksel M., Çetinel Ş., Yeǧen B.C., Şener G.
dc.date.accessioned2022-03-15T02:09:53Z
dc.date.available2022-03-15T02:09:53Z
dc.date.issued2013
dc.description.abstractIn this study we have investigated the possible protective effect of betulinic acid (BA) on colonic inflammation in rats. Colitis was induced in Sprague-Dawley rats of both sexes by intracolonic administration of 1 ml trinitrobenzene sulphonic acid (TNBS). Colitisinduced rats received orogastrically either betulinic acid (50 mg/kg/day) or vehicle (0.05% DMSO) for 3 days. At the 72nd hour of colitis induction, the rats were decapitated and trunk blood was collected for the measurement of TNF-α, IL-1Β, lactate dehydrogenase (LDH) levels and total antioxidant capacity (AOC). The distal 8 cm of colon were scored macroscopically, and the degree of oxidant damage was evaluated by malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase activity (MPO), collagen content and by histological analysis. Generation of oxidants was evaluated by tissue luminol and lucigenin chemiluminescences (CL). Colitis caused significant increases in the colonic CL values, macroscopic damage scores, MDA, MPO and collagen levels, along with a significant decrease in tissue GSH level. Similarly, serum TNF-α, IL-1Β, as well as LDH were elevated and AOC was reduced in the vehicle-treated colitis group as compared to control group. On the other hand, betulinic acid treatment reversed all these biochemical indices, as well as histopathological alterations induced by TNBS, suggesting that betulinic acid protects the colonic tissue via its radical scavenging and antioxidant activities.
dc.identifier.doi10.12991/201317393
dc.identifier.issn13090801
dc.identifier.urihttps://hdl.handle.net/11424/247337
dc.language.isoeng
dc.relation.ispartofMarmara Pharmaceutical Journal
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBetulinic acid
dc.subjectColitis
dc.subjectInflammation
dc.subjectOxidative damage
dc.subjectTrinitrobenzene sulphonic acid
dc.titleThe effect of betulinic acid on TNBS-induced experimental colitis [Betulinik asitin TNBS ile oluşturulan deneysel kolit üzerine etkileri]
dc.typearticle
dspace.entity.typePublication
local.avesis.id56dead87-b057-47f0-bfc8-192615358b9c
local.import.packageSS21
local.import.sourceScopus
local.indexed.atSCOPUS
oaire.citation.endPage59
oaire.citation.issue1
oaire.citation.startPage52
oaire.citation.titleMarmara Pharmaceutical Journal
oaire.citation.volume17
relation.isAuthorOfPublicatione4eaf9ac-f8dc-4e2b-b940-895cc906790d
relation.isAuthorOfPublication.latestForDiscoverye4eaf9ac-f8dc-4e2b-b940-895cc906790d

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