Publication: Kinetic analysis of the inhibitory effect of trichloroethylene (TCE) on nitrification in cometabolic degradation
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Date
2007
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SPRINGER
Abstract
In this study, the inhibitory effect of TCE on nitrification process was investigated with an enriched nitrifier culture. TCE was found to be a competitive inhibitor of ammonia oxidation and the inhibition constant (K-I) was determined as 666-802 mu g/l. The TCE affinity for the AMO enzyme was significantly higher than ammonium. The effect of TCE on ammonium utilization was evaluated with linearized plots of Monod equation (e.g., Lineweaver-Burk, Hanes-Woolf and Eadie-Hofstee plots) and non-linear least square regression (NLSR). No significant differences were found among these data evaluation methods in terms of kinetic parameters obtained.
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Keywords
cometabolism, kinetics, modelling, nitrification, trichloroethylene, GEL-ELECTROPHORESIS DGGE, NITRIFYING BACTERIA, IN-SITU, NITROSOMONAS-EUROPAEA, CHLORINATED SOLVENTS, MIXED CULTURE, METHANE, BIODEGRADATION, AMMONIA, TRANSFORMATION