Publication:
A RAPID AND SIMPLE ISOLATION OF ENDOTHELIAL-CELL GROWTH SUPPLEMENT FROM MOUSE-BRAIN

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dc.contributor.authorsSAHIN, S; AKOGLU, T; LAWRENCE, R; SEN, LS
dc.date.accessioned2022-03-13T23:23:05Z
dc.date.available2022-03-13T23:23:05Z
dc.date.issued1995
dc.description.abstractIn-vitro studies using endothelial cells are mainly performed using endothelial cell monolayer cultures (ECC) prepared from umblical cord vein endothelium and grown in tissue culture flasks. These cultures requires growth supplements to promote endothelial cell growth. A specific endothelial cell growth factor (ECGF) was extracted from bovine brain (1,2), gene has been cloned and the nucleotid sequence described (3). Commercially available ECGF for use as endothelial cell culture supplement however, is still produced for convenience from bovine brain. We have developed a simple and rapid method of EC growth supplement production which is considerably cheaper and as effective as the commercially available ECG supplements.
dc.identifier.doi10.1016/0049-3848(95)91523-N
dc.identifier.issn0049-3848
dc.identifier.pubmed7495102
dc.identifier.urihttps://hdl.handle.net/11424/238479
dc.identifier.wosWOS:A1995RE10800012
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofTHROMBOSIS RESEARCH
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectECGF
dc.subjectENDOTHELIAL CELL
dc.subjectGROWTH SUPPLEMENT
dc.subjectFIBROBLAST
dc.titleA RAPID AND SIMPLE ISOLATION OF ENDOTHELIAL-CELL GROWTH SUPPLEMENT FROM MOUSE-BRAIN
dc.typeother
dc.type.subnote
dspace.entity.typePublication
local.import.packageSS19
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages5
oaire.citation.endPage145
oaire.citation.issue1
oaire.citation.startPage141
oaire.citation.titleTHROMBOSIS RESEARCH
oaire.citation.volume79

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