Publication:
Vitreoscilla hemoglobin renders Enterobacter aerogenes highly susceptible to heavy metals

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dc.contributor.authorARMAN, AHMET
dc.contributor.authorsGeckil, H; Arman, A; Gencer, S; Ates, B; Yilmaz, HR
dc.date.accessioned2022-03-12T17:16:56Z
dc.date.available2022-03-12T17:16:56Z
dc.date.issued2004
dc.description.abstractWhen expressed in heterologous microorganisms Vitreoscilla hemoglobin (VHb) acts as oxygen storage and causes a higher oxygen uptake. In this study, the effect of this protein on growth, sensitivity and antioxidant properties of Enterobacter aerogenes exposed to metal stress was investigated. The strain expressing VHb was more sensitive to mercury and cadmium as the minimal inhibitory concentration (MIC) for these metals was up to 2-fold lower in this strain than the host and the recombinant strain carrying a comparable plasmid. At lower concentrations than MIC, the metals partially limited growth and caused an inhibition proportional to metal concentration applied. The growth pattern of VHb expressing strain was also distinctly different from other two non-hemoglobin strains. The hemoglobin containing strain showed substantially higher superoxide dismuates (SOD) activity than the non-hemoglobin strains, while catalase levels were similar in all strains. All strains exposed to copper, however, showed similar MIC values, growth patterns, and SOD and catalase levels.
dc.identifier.doi10.1007/s10534-004-1216-x
dc.identifier.eissn1572-8773
dc.identifier.issn0966-0844
dc.identifier.pubmed15689114
dc.identifier.urihttps://hdl.handle.net/11424/227728
dc.identifier.wosWOS:000226552000013
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofBIOMETALS
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectVitreoscilla hemoglobin
dc.subjectbacterial hemoglobin
dc.subjectoxidative stress
dc.subjectantioxidant systems
dc.subjectcatalase
dc.subjectsuperoxide dismutase
dc.subjectESCHERICHIA-COLI
dc.subjectSUPEROXIDE-DISMUTASE
dc.subjectCELL-GROWTH
dc.subjectRESISTANCE
dc.subjectBACTERIA
dc.subjectMERCURY
dc.subjectEXPRESSION
dc.subjectIRON
dc.subjectGENE
dc.titleVitreoscilla hemoglobin renders Enterobacter aerogenes highly susceptible to heavy metals
dc.typearticle
dspace.entity.typePublication
local.avesis.id63cb8684-b0b2-4804-8259-5640d58a780a
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages9
oaire.citation.endPage723
oaire.citation.issue6
oaire.citation.startPage715
oaire.citation.titleBIOMETALS
oaire.citation.volume17
relation.isAuthorOfPublicationaeb6705c-76c3-423f-bf49-bf3e1bb0edf1
relation.isAuthorOfPublication.latestForDiscoveryaeb6705c-76c3-423f-bf49-bf3e1bb0edf1

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