Publication: Correlation between plasma ccfDNA, mtDNA changes, CTCs, and epithelial-mesenchymal transition in breast cancer patients undergoing NACT
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Date
2024-01-01
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Abstract
Background/aim: Breast cancer is the most prevalent cancer in women, emphasizing need for noninvasive blood biomarkers to aid in
treatment selection. Previous studies have demonstrated elevated levels of plasma circulating cell-free DNA (ccfDNA) in breast cancer
patients. Both ccfDNA and mitochondrial DNA (mtDNA) are fragments released into the bloodstream. In this study, we investigated
effectiveness of ccfDNA and mtDNA as indicators of treatment response and explored their potential as monitoring biomarkers.
Additionally, we compared these markers with circulating tumor cell (CTC) data and assessed their relationship with epithelialmesenchymal
transition (EMT).
Materials and methods: Thirty-six female breast cancer patients and 21 healthy females were included in the study. Quantitative
polymerase chain reaction (qPCR) was performed on plasma samples to measure levels of ND1, ND4, ALU115, ALU247, and GAPDH,
and DNA integrity was determined by calculating ratios of ALU247/ALU115 and ND4/ND1.
Results: After treatment, patients had a significant decrease in ccfDNA levels and a significant increase in mtDNA copy number
(mtDNAcn). However, there was no significant change in ccfDNA and mtDNA integrity. When comparing all groups, patients exhibited
higher levels of ALU115 and ALU247 compared to controls. Moreover, patients demonstrated significantly lower ccfDNA integrity than
controls.
Conclusion: This study represents the first comprehensive investigation of plasma ccfDNA levels, mtDNAcn, and integrities collectively.
Furthermore, it is the first study to explore the relationship between these markers and CTCs, cancer stem cell markers, treatment
response, and metastatic status. Our findings suggest that plasma ccfDNA and mtDNA may serve as potential biomarkers for assessing
chemotherapy response and can be employed alone or in combination with other biomarkers to monitor treatment efficacy in breast
cancer patients.
Key words: Breast cancer, ccfDNA, mtDNA, neoadjuvant therapy, EMT
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Keywords
Tıp, Sağlık Bilimleri, Temel Tıp Bilimleri, Medicine, Health Sciences, Fundamental Medical Sciences, Klinik Tıp (Med), Klinik Tıp, Tıp Genel & Dahili, Clinical Medicine (Med), Clinical Medicine, Medicine General & Internal, Genel Tıp, General Medicine, Breast cancer, ccfDNA, EMT, mtDNA, neoadjuvant therapy
Citation
Çelik B., PEKER EYÜBOĞLU İ., Koca S., Ümit Uğurlu M., Alan Ö., GÜLLÜ AMURAN G., AKIN TELLİ T., Yumuk F., AKKİPRİK M., "Correlation between plasma ccfDNA, mtDNA changes, CTCs, and epithelial-mesenchymal transition in breast cancer patients undergoing NACT", Turkish Journal of Medical Sciences, cilt.54, sa.4, ss.652-665, 2024