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Metabolic engineering of Corynebacterium glutamicum for L-tyrosine production from glucose and xylose

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsKurpejović E., Burgardt A., Bastem G. M., Junker N., Wendisch V. F., SARIYAR AKBULUT B.
dc.date.accessioned2023-01-16T06:47:30Z
dc.date.available2023-01-16T06:47:30Z
dc.date.issued2023-02-10
dc.description.abstract© 2023 Elsevier B.V.Microbial production of aromatic compounds is an attractive and sustainable biotechnological approach. With this motivation, here metabolic engineering of Corynebacterium glutamicum for L-tyrosine (L-Tyr) overproduction was attempted by pushing the carbon flux more towards L-Tyr. Translational start codon exchanges of prephenate dehydratase (pheA), anthranilate synthase (trpE), and phenylalanine aminotransferase (pat) genes revealed that reduced expression of pheA was the major contributor to increased L-Tyr titer while codon exchange in trpE was effective to a lower extent. Overexpression of aroE and qsuC, encoding shikimate dehydrogenase and 3-dehydroquinate dehydratase, respectively, and of dapC (cg1253), which is predicted to encode prephenate aminotransferase, were futile to increase L-Tyr titer. Similarly, deletion of the qsuABD gene cluster had also not enhanced titer. As for increasing precursor supply, deletion of ptsG of glucose uptake and overexpression of inositol permease (iolT2) and glucokinase (glcK) were not effective, but with utilization of xylose, enabled by overexpression of xylose isomerase (xylA) and xylulokinase (xylB), titer improved. Highest L-Tyr titer using the construct was 3.1 g/L on glucose and 3.6 g/L on a 1:3 (w/v) mixture of glucose and xylose. This result displays the potential of the constructed strain to produce L-Tyr from lignocellulosic renewable carbon sources.
dc.identifier.citationKurpejović E., Burgardt A., Bastem G. M., Junker N., Wendisch V. F., SARIYAR AKBULUT B., "Metabolic engineering of Corynebacterium glutamicum for L-tyrosine production from glucose and xylose", Journal of Biotechnology, cilt.363, ss.8-16, 2023
dc.identifier.doi10.1016/j.jbiotec.2022.12.005
dc.identifier.endpage16
dc.identifier.issn0168-1656
dc.identifier.startpage8
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85145783089&origin=inward
dc.identifier.urihttps://hdl.handle.net/11424/285258
dc.identifier.volume363
dc.language.isoeng
dc.relation.ispartofJournal of Biotechnology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiyomedikal Mühendisliği
dc.subjectYaşam Bilimleri
dc.subjectBiyoteknoloji
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectBiomedical Engineering
dc.subjectLife Sciences
dc.subjectBiotechnology
dc.subjectNatural Sciences
dc.subjectEngineering and Technology
dc.subjectMühendislik, Bilişim ve Teknoloji (ENG)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMühendislik
dc.subjectMikrobiyoloji
dc.subjectBİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
dc.subjectMÜHENDİSLİK, BİYOMEDİKAL
dc.subjectEngineering, Computing & Technology (ENG)
dc.subjectLife Sciences (LIFE)
dc.subjectENGINEERING
dc.subjectMICROBIOLOGY
dc.subjectBIOTECHNOLOGY & APPLIED MICROBIOLOGY
dc.subjectENGINEERING, BIOMEDICAL
dc.subjectBiyomühendislik
dc.subjectFizik Bilimleri
dc.subjectUygulamalı Mikrobiyoloji ve Biyoteknoloji
dc.subjectBioengineering
dc.subjectPhysical Sciences
dc.subjectApplied Microbiology and Biotechnology
dc.subjectCorynebacterium glutamicum
dc.subjectL-Tyrosine overproduction
dc.subjectRational design
dc.subjectSustainable process
dc.titleMetabolic engineering of Corynebacterium glutamicum for L-tyrosine production from glucose and xylose
dc.typearticle
dspace.entity.typePublication
local.avesis.id89b44a5a-81eb-43c1-9c48-340f86a007b8
local.indexed.atPUBMED
local.indexed.atSCOPUS
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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