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An OMIC approach to elaborate the antibacterial mechanisms of different alkaloids

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dc.contributor.authorSARIYAR AKBULUT, BERNA
dc.contributor.authorsAvci, Fatma Gizem; Sayar, Nihat Alpagu; Akbulut, Berna Sariyar
dc.date.accessioned2022-03-12T22:25:31Z
dc.date.available2022-03-12T22:25:31Z
dc.date.issued2018
dc.description.abstractPlant-derived substances have regained interest in the fight against antibiotic resistance owing to their distinct antimicrobial mechanisms and multi-target properties. With the recent advances in instrumentation and analysis techniques, OMIC approaches are extensively used for target identification and elucidation of the mechanism of phytochemicals in drug discovery. In the current study, RNA sequencing based transcriptional profiling together with global differential protein expression analysis was used to comparatively elaborate the activities and the effects of the plant alkaloids boldine, bulbocapnine, and roemerine along with the well-known antimicrobial alkaloid berberine in Bacillus subtilis cells. The transcriptomic findings were validated by qPCR. Images from scanning electron microscope were obtained to visualize the effects on the whole-cells. The results showed that among the three selected alkaloids, only roemerine possessed antibacterial activity. Unlike berberine, which is susceptible to efflux through multidrug resistance pumps, roemerine accumulated in the cells. This in turn resulted in oxidative stress and building up of reactive oxygen species, which eventually deregulated various pathways such as iron uptake. Treatment with boldine or bulbocapnine slightly affected various metabolic pathways but has not changed the growth patterns at all. (C) 2018 Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.phytochem.2017.12.023
dc.identifier.issn0031-9422
dc.identifier.pubmed29494814
dc.identifier.urihttps://hdl.handle.net/11424/234934
dc.identifier.wosWOS:000430778200011
dc.language.isoeng
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofPHYTOCHEMISTRY
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAporphine alkaloid
dc.subjectRoemerine
dc.subjectAntibacterial
dc.subjectRNA-Seq
dc.subjectProteomics
dc.subjectBacillus subtilis
dc.subjectBACILLUS-SUBTILIS
dc.subjectGENE-EXPRESSION
dc.subjectMULTIDRUG-RESISTANCE
dc.subjectESCHERICHIA-COLI
dc.subjectOXIDATIVE STRESS
dc.subjectIRON
dc.subject(-)-ROEMERINE
dc.subjectPROFILES
dc.titleAn OMIC approach to elaborate the antibacterial mechanisms of different alkaloids
dc.typearticle
dspace.entity.typePublication
local.avesis.id081c765d-7284-418a-a32c-e9432748343a
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages9
local.journal.quartileQ1
oaire.citation.endPage131
oaire.citation.startPage123
oaire.citation.titlePHYTOCHEMISTRY
oaire.citation.volume149
relation.isAuthorOfPublicationa9f127d3-8332-44dd-a532-34f3ef20bdb5
relation.isAuthorOfPublication.latestForDiscoverya9f127d3-8332-44dd-a532-34f3ef20bdb5

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