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Neuroprotective Effects of Alpha-Lipoic Acid in Experimental Spinal Cord Injury in Rats

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dc.contributor.authorVELİOĞLU ÖĞÜNÇ, AYLİZ
dc.contributor.authorsToklu, Hale Z.; Hakan, Tayfun; Celik, Hasan; Biber, Necat; Erzik, Can; Ogunc, Ayliz V.; Akakin, Dilek; Cikler, Esra; Cetinel, Sule; Ersahin, Mehmet; Sener, Goksel
dc.date.accessioned2022-03-14T10:06:33Z
dc.date.available2022-03-14T10:06:33Z
dc.date.issued2010-01
dc.description.abstractBackground: Oxidative stress is a mediator of secondary injury to the spinal cord following trauma. Objective: To investigate the putative neuroprotective effect of a-lipoic acid (LA), a powerful antioxidant, in a rat model of spinal cord injury (SCI). Methods: Wistar albino rats were divided as control, vehicle-treated SCI, and LA-treated SCI groups. To induce SCI, a standard weight-drop method that induced a moderately severe injury (100 g/cm force) at T10 was used. Injured animals were given either 50 mg/kg LA or saline at 30 minutes postinjury by intraperitoneal injection. At 7 days postinjury, neurologic examination was performed, and rats were decapitated. Spinal cord samples were taken for histologic examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity, and DNA fragmentation. Formation of reactive oxygen species in spinal cord tissue samples was monitored by using a chemiluminescence (CL) technique. Results: SCI caused a significant decrease in spinal cord GSH content, which was accompanied with significant increases in luminol CL and MDA levels, MPO activity, and DNA damage. Furthermore, LA treatment reversed all these biochemical parameters as well as SO-induced histopathologic alterations. Conversely, impairment of the neurologic function caused by SCI remained unchanged. Conclusion: The present study suggests that LA reduces SCI-induced oxidative stress and exerts neuroprotection by inhibiting lipid peroxidation, glutathione depletion, and DNA fragmentation.
dc.identifier.doi10.1080/10790268.2010.11689719
dc.identifier.eissn2045-7723
dc.identifier.issn1079-0268
dc.identifier.pubmed21061900
dc.identifier.urihttps://hdl.handle.net/11424/244055
dc.identifier.wosWOS:000283705200010
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS LTD
dc.relation.ispartofJOURNAL OF SPINAL CORD MEDICINE
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAlpha-lipoic acid
dc.subjectAntioxidants
dc.subjectSpinal cord injuries
dc.subjectTrauma
dc.subjectNeuroprotection
dc.subjectLipid Peroxidation
dc.subjectGlutathione, Myeloperoxidase
dc.subjectDNA damage
dc.subjectISCHEMIA-REPERFUSION INJURY
dc.subjectTRAUMATIC BRAIN-INJURY
dc.subjectOXIDATIVE STRESS
dc.subjectDIHYDROLIPOIC ACID
dc.subjectANTIOXIDANT
dc.subjectMETHYLPREDNISOLONE
dc.subjectGLUTATHIONE
dc.subjectPROTECTS
dc.titleNeuroprotective Effects of Alpha-Lipoic Acid in Experimental Spinal Cord Injury in Rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id92600323-05e5-4435-b5f8-54010ed0b48f
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.numberofpages9
oaire.citation.endPage409
oaire.citation.issue4
oaire.citation.startPage401
oaire.citation.titleJOURNAL OF SPINAL CORD MEDICINE
oaire.citation.volume33
relation.isAuthorOfPublication13300bf6-ba96-4f87-9868-b0d2c86f572a
relation.isAuthorOfPublication.latestForDiscovery13300bf6-ba96-4f87-9868-b0d2c86f572a

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