Publication: Cerebellum and oxidative stress in natural and accelerated aging model
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Date
2023-01-05
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Abstract
AIM: Intracerebroventricular administration of galactose causes motor coordination deficiency by decreasing glutathione (GSH) level in the cerebellum. It has been shown that aging increases oxidative stress and Sirtuin 2 (Sirt2) expression in rat cerebellum tissue and Sirt2 inhibition has a protective effect in aging. In our study, we aimed to investigate the effect of AGK-2 administration, a specific Sirt2 inhibitor, on oxidative stress in an accelerated aging model with natural and D-galactose (D-GAL) administration.METHODS: In the study, 7 groups were formed using 48 male rats of Wistar (W) and Sprague-Dawley (SD) species;1) Young-Control (3 months, n=6), 2) Young-AGK-2 (3 months, n=6), 3) Old-Control (22 months, n=6), 4) Old-AGK-2 (22 months, n=6), 5) D-GAL (3 months, n=9), 6) Solvent+D-GAL (3 months, n=8), 7) Solvent+D-GAL+AGK-2 (3 months, n=7). Control groups were given 4% DMSO+PBS, and experimental groups were given AGK-2 (10 μM/bw) subcutaneously (SC). For the accelerated aging model, D-galactose (150 mg/kg/day, SC) was administered for 10 weeks. Malondialdehyde (MDA) and GSH levels in cerebellum tissue were measured by spectrophotometric method.In the statistical analysis, one-way ANOVA (post-hoc LSD) was used to determine the differences between groups. The statistical significance level was set at p<0.05.RESULTS: The D-GAL administration increased the cerebellum MDA level significantly compared to the young control group (p<0.001). In the D-GAL group, AGK-2 administration decreased the MDA levels and increased the GSH levels (p=0.003; p=0.006). D-GAL administration increased MDA levels more and decreased GSH levels significantly compared to aged rats (p=0.006; p<0.001). AGK-2 administration in natural aging was found to be more effective in increasing GSH levels compared to the accelerated aging model (p<0.001).CONCLUSION: Both models compared increased oxidant stress in the cerebellum. AGK-2 application was found to be more effective than D-GAL on oxidant stress in natural aging.Keywords:AGK-2, Accelerated aging, Cerebellum, D-galactose, Natural aging, Oxidative Stress
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Tıp, Sağlık Bilimleri, Medicine, Health Sciences, Klinik Tıp (MED), Klinik Tıp, Clinical Medicine (MED), CLINICAL MEDICINE, AGK-2, Accelerated aging, Cerebellum, D-galactose, Natural aging, Oxidative Stress.
Citation
Kavak H., Bahar A. N., Keskin Aktan A., Akbulut K. G., \"Cerebellum and Oxidative Stress in Natural and Accelerated Aging Model\", 47th Turkish Physiology Congress, Antalya, Türkiye, 1 - 04 Kasım 2022, cilt.237, sa.727, ss.61