Publication: Neuropeptide-w protects against stress-induced gastric ulcer in rats via the involvement of capsaicin-sensitive vagal afferent fibers
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Date
2020-11-28
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Abstract
AIM: Neuropeptide-W (NPW), expressed in
hypothalamus and peripheral organs, activates
hypothalamus-pituitary-adrenal axis, and may have a
physiological role in neuroendocrine response to stress.
The aim was to evaluate protective effects of NPW on
stress-induced gastric injury in rats.
METHODS: Sprague-Dawley male rats were fasted for
24 hours, restrained and immersed in water-bath for 6 h
to induce water-immersion restraint stress (WIRS), and
NPW (0.1 or 5µg/kg) or saline was injected
subcutaneously at 15 minutes before WIRS (n=24),
while saline-injected control rats had no WIRS (n=8).
For degeneration of vagal afferent fibers (VAD), in
some rats (n=24) capsaicin (1%) was applied
perivagally under ketamine anesthesia, and 3 weeks
later WIRS was induced. Using a laser Doppler, gastric
serosal blood flow was monitored under anesthesia.
Following cardiac puncture, gastric tissues were
removed for macroscopic/microscopic scoring and
measurement of myeloperoxidase activity,
malondialdehyde and glutathione levels. Gastric NF-κB
and cerebral NPW mRNA expressions were detected by
RT-PCR. One-way ANOVA was used for statistical
analysis.
RESULTS: WIRS decreased mean serosal blood flow,
resulted in elevated macroscopic/ microscopic scores
compared to control group (p<0.001), while
myeloperoxidase activity and malondialdehyde level
were elevated (p<0.05) and antioxidant glutathione was depleted (p<0.001). WIRS depressed gastric NF-κB and
cerebral NPW mRNA expressions (p<0.01). Neither
doses of NPW changed gastric NF-κB mRNA. Lowerdose of NPW elevated blood flow (p<0.001), abolished
WIRS-induced elevations in myeloperoxidase and
malondialdehyde levels (p<0.05). High-dose NPW
replenished gastric glutathione and brain NPW
expression and reduced scores (p<0.05-0.01). Despite
that VAD did not alter effects of high-dose NPW,
reductions in malondialdehyde and myeloperoxidase,
and improvement in blood flow by low-dose NPW were
abolished by VAD (p<0.05).
CONCLUSION: In stress-induced oxidative gastric
injury, NPW provides gastroprotection by improving
depressed blood flow and inhibiting ulcer-induced
oxidative injury, which involve contribution of vagal
afferent fibers
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Keywords
Neuropeptide-W, gastric ulcer, waterimmersion restraint stress, oxidative damage, inflammation
Citation
ARABACI TAMER S., AKBULUT S., AKAKIN D., PEKER EYÜBOĞLU İ., AKKİPRİK M., YEGEN B., \"Neuropeptide-W Protects Against Stress-Induced Gastric Ulcer in Rats via the Involvement of Capsaicin-Sensitive Vagal Afferent Fibers\", 4th International Congress of Turkish Neuroendocrinology Society, Türkiye, 26 - 28 Kasım 2020