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Evaluation of Gadolinium Pre-Treatment with or without Splenectomy in the Setting of Renal Ischemia Reperfusion Injury in Rats

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dc.contributor.authorATA, PINAR
dc.contributor.authorsKara, Melih; Tellioglu, Gurkan; Sehirli, Ozer; Yildar, Murat; Krand, Osman; Berber, Ibrahim; Cetinel, Sule; Eren, P. A.; Sener, Goeksel; Titiz, Izzet
dc.date.accessioned2022-03-12T17:37:53Z
dc.date.available2022-03-12T17:37:53Z
dc.date.issued2009
dc.description.abstractIntroduction. This study aims to investigate gadolinium chloride (Gd) pre-treatment with/without splenectomy (Splx) in the setting of renal ischemia/reperfusion (IR) injury in rats. Materials and Methods. Under anesthesia, male Wistar albino rats with or without splenectomized (Splx) were right nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 3 h of reperfusion. Gadolinium chloride (10 mg kg(-1)) or saline was administered 24 hours prior to ischemia via penile vein. Right nephrectomy and intravenous saline administration was performed in the control group. At the end of the reperfusion period, following decapitation, kidney samples were taken for histological examination or determination of renal malondialdehyde (MDA) and glutathione (GSH) levels and myeloperoxidase (MPO) and Na+-K+ ATPase activities. Creatinine, blood urea nitrogen (BUN), lactate dehydrogenase (LDH), TNF-alpha, and IL-1 beta were assayed in the serum samples. Results. Ischemia/reperfusion caused significant increases in the serum TNF-alpha, IL-1 beta, BUN, creatinine, AST, ALT, LDH, and tissue MDA levels and MPO activity, while either Gd pre-treatment or Splx decreased these parameters significantly. On the other hand, IR induced a decrease in the tissue GSH, and Na+-K+ ATPase activity was restored by both gadolinium and Splx. Furthermore, histopathological alterations induced by IR were also reversed. Conclusion. The extent of renal IR injury depends on the pro-inflammatory cytokine response. Gd pretreatment decreases macrophage-derived cytokine secretion and thereby effectively limits the extent of renal IR injury in rats similar to Splx. Further studies needed to define an optimal way of decreasing macrophage-derived cytokine release due to the clinical limitations of Gd.
dc.identifier.doi10.3109/08860220903216162
dc.identifier.eissn1525-6049
dc.identifier.issn0886-022X
dc.identifier.pubmed20030532
dc.identifier.urihttps://hdl.handle.net/11424/229426
dc.identifier.wosWOS:000273988000014
dc.language.isoeng
dc.publisherTAYLOR & FRANCIS LTD
dc.relation.ispartofRENAL FAILURE
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectrenal ischemia reperfusion
dc.subjectgadolinium chloride
dc.subjectsplenectomy
dc.subjectpro-inflammatory cytokine
dc.subjectINTESTINAL ISCHEMIA
dc.subjectISCHEMIA/REPERFUSION INJURY
dc.subjectCONTRAST AGENTS
dc.subjectFREE-RADICALS
dc.subjectNITRIC-OXIDE
dc.subjectFAILURE
dc.subjectLIVER
dc.subjectKIDNEY
dc.subjectMACROPHAGES
dc.subjectGLUTATHIONE
dc.titleEvaluation of Gadolinium Pre-Treatment with or without Splenectomy in the Setting of Renal Ischemia Reperfusion Injury in Rats
dc.typearticle
dspace.entity.typePublication
local.avesis.id50c0400c-b6f7-4779-97d2-437079d83fc4
local.import.packageSS17
local.indexed.atWOS
local.indexed.atSCOPUS
local.journal.numberofpages8
oaire.citation.endPage963
oaire.citation.issue10
oaire.citation.startPage956
oaire.citation.titleRENAL FAILURE
oaire.citation.volume31
relation.isAuthorOfPublication4ef620f9-0462-4687-84fa-c983fb788878
relation.isAuthorOfPublication.latestForDiscovery4ef620f9-0462-4687-84fa-c983fb788878

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