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Quercetin-Induced Cell Death in Human Papillary Thyroid Cancer (B-CPAP) Cells

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dc.contributor.authorYALÇIN, AHMET SUHA
dc.contributor.authorsAltundag, Ergul Mutlu; Kasaci, Tolga; Yilmaz, Ayse Mine; Karademir, Betul; Kocturk, Semra; Taga, Yavuz; Yalcin, A. Suha
dc.date.accessioned2022-03-14T08:15:58Z
dc.date.available2022-03-14T08:15:58Z
dc.date.issued2016
dc.description.abstractIn this study, we have investigated the antiproliferative effect of quercetin on human papillary thyroid cancer cells and determined the apoptotic mechanisms underlying its actions. We have used different concentrations of quercetin to induce apoptosis and measured cell viability. Apoptosis and cell cycle analysis was determined by flow cytometry using Annexin V and propidium iodide. Finally, we have measured changes in caspase-3 and cleaved poly(ADP-ribose) polymerase (PARP) protein expression levels as hallmarks of apoptosis and Hsp90 protein expression level as a marker of proteasome activity in treated and control cells. Quercetin treatment of human papillary thyroid cancer cells resulted in decreased cell proliferation and increased rate of apoptosis by caspase activation. Furthermore, it was demonstrated that quercetin induces cancer cell apoptosis by downregulating the levels of Hsp90. In conclusion, we have shown that quercetin induces downregulation of Hsp90 expression that may be involved in the decrease of chymotrypsin-like proteasome activity which, in order, induces inhibition of growth and causes cell death in thyroid cancer cells. Thus, quercetin appears to be a promising candidate drug for Hsp90 downregulation and apoptosis of thyroid cancer cells.
dc.identifier.doi10.1155/2016/9843675
dc.identifier.eissn2042-0072
dc.identifier.issn2090-8067
dc.identifier.pubmed27057371
dc.identifier.urihttps://hdl.handle.net/11424/241346
dc.identifier.wosWOS:000369485900001
dc.language.isoeng
dc.publisherHINDAWI LTD
dc.relation.ispartofJOURNAL OF THYROID RESEARCH
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDIETARY FLAVONOIDS
dc.subjectAPOPTOSIS
dc.subjectINHIBITION
dc.subjectPOLYPHENOLS
dc.subjectBIOAVAILABILITY
dc.subjectPROLIFERATION
dc.subjectCHAPERONES
dc.subjectSTRATEGIES
dc.subjectPATHWAYS
dc.subjectPROTEINS
dc.titleQuercetin-Induced Cell Death in Human Papillary Thyroid Cancer (B-CPAP) Cells
dc.typearticle
dspace.entity.typePublication
local.avesis.idd0bdb3b8-1a07-4648-b645-13b2e443d197
local.import.packageSS16
local.indexed.atWOS
local.indexed.atSCOPUS
local.indexed.atPUBMED
local.journal.articlenumber9843675
local.journal.numberofpages10
oaire.citation.titleJOURNAL OF THYROID RESEARCH
oaire.citation.volume2016
relation.isAuthorOfPublication7a2bc583-aab6-43e7-9cc3-0d7a60773409
relation.isAuthorOfPublication.latestForDiscovery7a2bc583-aab6-43e7-9cc3-0d7a60773409

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