Person: ERTAŞ, BÜŞRA
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Publication Open Access Effect of riboflavin on rat bladder contractility and oxidant damage following ischemia/reperfusion(2023-01-01) ERTAŞ, BÜŞRA; ERTAŞ B.Ischemia/reperfusion (IR) encompasses the processes of organ function damage and oxidative injury depending on the successive blood flow obstruction and removal of the obstruction. Known as a vitamin, riboflavin (Rb) is known to be protective against tissue damage with its antioxidant and anti-inflammatory properties. The aim of this study is to investigate the effect of Rb treatment on bladder contraction dysfunction and tissue damage due to IR. The study was conducted with forty Sprague-Dawley rats. The abdominal aorta of anaesthetized rats was occluded to induce ischemia (60 min) and then allowed reperfusion (60 min). Rb (25 mg/kg), n-acetylcysteine NAC (100 mg/kg) or saline was administered orally 15 min before the IR model immediately. The bladder was assessed by biochemical and histological analysis, and the contractility of the I/R-related bladder was detected by organ bath. Compared to the control group, MDA, MPO and caspase-3 activities increased in the IR group, while GSH levels decreased. MDA, GSH and caspase-3 activities were reversed with Rb treatment, but there was no change in MPO level. Healing of IR-induced edema and oxidant damage with Rb and NAC treatment resulted in improvement of the thinning of the bladder wall. According to these results, it can be said that Rb therapy can regulate IR-induced bladder dysfunction by improving antioxidant properties and tissue damage.Publication Metadata only The effect of myrtus communis l. extract on nephrolithiasis model in rats(2023-01-01) ERTAŞ, BÜŞRA; ŞEN, ALİ; ŞENER, TARIK EMRE; ERTAŞ B., Dorucu D., Gulerturk O., ŞEN A., ÇEVİK Ö., Cetinel S., Eker P., Akgun A., ŞENER T. E., ŞENER G.Objective: Nephrolithiasis is a common urological disease that can lead to renal failure. Oxidative stress has been shown to be a contributing factor for nephrolithiasis and many agents have been studied to prevent and treat oxidative stress-related nephrolithiasis and renal damage. Myrtus communis (MC) extract has been shown to be an important antioxidant in different animal models. In this study, myrtus communis (MC) extract was administered preventively or therapeutically to rats with kidney stones, and its effectiveness was investigated. Materials and metods: Wistar albino rats were divided into 4 groups (n=8); control (C), ethylene glycol (EG), EG+preventive MC, and EG+curative MC groups. The nephrolithiasis model was created by adding 0.75% EG to drinking water for 8 weeks. Ultimately, 24-hour urine was collected to measure calcium, citrate, and creatinine levels. After decapitation, kidney tissues were harvested for histological analyses, measurement of osteopontin and 8-hydroxydeoxyguanosine (8-OHdG) levels, and N-acetyl-β-glucosaminidase (NAG), myeloperoxidase (MPO) and caspase-3 activities. Results: In 24-hour urine samples, calcium, citrate and creatinine levels were decreased in the EG group, while oxalate levels were increased and in treatment groups these parameters returned to control levels. MPO, 8-OHdG, caspase-3 and NAG activity were significantly increased in tissue and these changes were reversed in both MC groups. Histological findings also supported the biochemical parameters. Conclusion: Myrtus communis can reduce oxidative stress and histopathological changes in kidney tissues in rat nephrolithiasis model when used as either a preventive or therapeutic agent. If supported with further clinical trials, MC might have clinical implications in preventing oxidative renal cell injury and ultimately kidney stone formation.Publication Open Access Apocynin exhibits an ameliorative effect on endothelial dysfunction/atherosclerosis-related factors in high-fat diet-induced obesity in rats(2024-05-31) ERTAŞ, BÜŞRA; KOÇYİĞİT SEVİNÇ, SEVGİ; ERCAN, FERİHA; ORUN, OYA; AKKİPRİK, MUSTAFA; ÇETİNEL, ŞULE; Bulbul Ayci N., ERTAŞ B., Keles Kaya R., Kocyigit Sevinc S., Amuran G. G., ERCAN F., Sener G., ORUN O., AKKİPRİK M., ÇETİNEL Ş.Objective: The aim of this study was to reveal the effect of apocynin (APO) on the factors involved in obesity-related endothelial dysfunction (ED) and atherosclerosis (AS). Materials and Methods: Male Wistar albino rats were divided into control (CNT), high-fat diet (HFD) and HFD+APO groups. HFD and HFD+APO groups were fed HFD for sixteen weeks. APO (25 mg/kg) was administered to the HFD+APO group for the last four weeks. The effects of APO on: AS-related metabolic parameters (triglyceride, total cholesterol, high-density lipoprotein-cholesterol, insulin and leptin), oxidative stress (OS), [ malondialdehyde, glutathione, nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase-2, oxidised-low-density lipoprotein (ox-LDL) and 8-hydroxy-2-deoxyguanosine], low-density lipoprotein and ox-LDL uptake potential (activin receptor-like kinase-1 and lectin-like oxidized low-density lipoprotein receptor-1, respectively), tissue inflammation (myeloperoxidase, monocyte-chemoattractant-protein-1, tumor necrosis factor-alpha), ED (endothelial-nitric oxide synthase, inducible-nitric oxide synthase, nitric oxide), programmed cell death (terminal deoxynucleotidyl-transferase-dUTP-nick-end labeling, cleaved-poly-ADP-ribose-polymerase, gasdermin-D N-terminal fragment, caspase-1), smooth muscle cell transformation (alpha-smooth muscle actin), histology and ultrastructure of thoracic aorta were evaluated. Results: In obesity, APO had an ameliorative effect on metabolic parameters, OS, inflammation, ED, programmed cell death and oxLDL uptake potential, but not on foam cell formation and LDL uptake potential. Conclusion: Apocynin may improve ED and AS in obesity by suppressing OS-linked factors involved in the early stage of AS.