Publication: Apocynin exhibits an ameliorative effect on endothelial dysfunction/atherosclerosis-related factors in high-fat diet-induced obesity in rats
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Date
2024-05-31
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Abstract
Objective: The aim of this study was to reveal the effect of apocynin (APO) on the factors involved in obesity-related endothelial dysfunction (ED) and atherosclerosis (AS). Materials and Methods: Male Wistar albino rats were divided into control (CNT), high-fat diet (HFD) and HFD+APO groups. HFD and HFD+APO groups were fed HFD for sixteen weeks. APO (25 mg/kg) was administered to the HFD+APO group for the last four weeks. The effects of APO on: AS-related metabolic parameters (triglyceride, total cholesterol, high-density lipoprotein-cholesterol, insulin and leptin), oxidative stress (OS), [ malondialdehyde, glutathione, nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase-2, oxidised-low-density lipoprotein (ox-LDL) and 8-hydroxy-2-deoxyguanosine], low-density lipoprotein and ox-LDL uptake potential (activin receptor-like kinase-1 and lectin-like oxidized low-density lipoprotein receptor-1, respectively), tissue inflammation (myeloperoxidase, monocyte-chemoattractant-protein-1, tumor necrosis factor-alpha), ED (endothelial-nitric oxide synthase, inducible-nitric oxide synthase, nitric oxide), programmed cell death (terminal deoxynucleotidyl-transferase-dUTP-nick-end labeling, cleaved-poly-ADP-ribose-polymerase, gasdermin-D N-terminal fragment, caspase-1), smooth muscle cell transformation (alpha-smooth muscle actin), histology and ultrastructure of thoracic aorta were evaluated. Results: In obesity, APO had an ameliorative effect on metabolic parameters, OS, inflammation, ED, programmed cell death and oxLDL uptake potential, but not on foam cell formation and LDL uptake potential. Conclusion: Apocynin may improve ED and AS in obesity by suppressing OS-linked factors involved in the early stage of AS.
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Tıp, Sağlık Bilimleri, Temel Tıp Bilimleri, Medicine, Health Sciences, Fundamental Medical Sciences, Klinik Tıp (MED), Klinik Tıp, TIP, GENEL & DAHİLİ, Clinical Medicine (MED), CLINICAL MEDICINE, MEDICINE, GENERAL & INTERNAL, Genel Tıp, General Medicine, Apocynin, Atherosclerosis, Endothelial dysfunction, High-fat diet, Obesity, Oxidative stress
Citation
Bulbul Ayci N., ERTAŞ B., Keles Kaya R., Kocyigit Sevinc S., Amuran G. G., ERCAN F., Sener G., ORUN O., AKKİPRİK M., ÇETİNEL Ş., "Apocynin exhibits an ameliorative effect on endothelial dysfunction/atherosclerosis-related factors in high-fat diet-induced obesity in rats", Marmara Medical Journal, cilt.37, sa.2, ss.238-247, 2024