Person: AYDİN, YILDIZ
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AYDİN
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YILDIZ
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Publication Metadata only An EST-SSR marker linked with yellow rust resistance in wheat(ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY, 2010) AYDİN, YILDIZ; Ercan, S.; Ertugrul, F.; Aydin, Y.; Akfirat, F. S.; Hasancebi, S.; Akan, K.; Mert, Z.; Bolat, N.; Yorgancilar, O.; Altinkut-Uncuoglu, A.Expressed sequenced tags containing simple sequence repeats (EST-SSRs) were used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.). A cross between yellow rust resistant (PI178383) and susceptible (Harmankaya99) wheat genotypes was performed and respective DNA pools from the resistant and susceptible F-2 seedlings were constructed. 78 EST-SSR primers were used for bulked segregant analysis and one EST-SSR marker (Pk54), identified as 200 bp fragment, was present in the resistant parent and resistant F-2 hybrids but not in the susceptible ones. 108 wheat genotypes differing in yellow rust resistance were screened with Pk54 and 68 % of the wheat genotypes, known to be yellow rust resistant, had the Pk54 marker, further suggesting that the presence of this marker correlates with yellow rust resistance.Publication Metadata only An assessment on the floristic variety of Colchicum genus of Turkey in terms of morphological characteristics(ELSEVIER, 2021) ALTINKUT UNCUOĞLU, AHU; Sahin, Ezgi Cabuk; Kaya, Erdal; Tuyel, Umut; Aydin, Yildiz; Ozhatay, Neriman; Uncuoglu, Ahu AltinkutThe study seeks to identify Colchicum species characterizing the morphological traits and to group the characters into species-specific clusters using Principal Component Analysis (PCA). Fifty-two Colchicum L. species, 25 of which are endemic, were characterized with important 36 morphological traits (21 for flower, 11 for leaf, 2 for corm and 2 for capsule). As a result of the morphological measurements, Colchicum technical certificate based on UPOV (The International Union for the Protection of New Varieties of Plants) was prepared and colour catalogue was created based on flowering time, perigon segments, perigon tube, anther, leaf, corm and capsule characters indicating the vast morphological differences among Colchicum species representing Turkish Flora. After the evaluation of measurements by PCA based on 36 morphological variables, it was observed that there is a clear separation between species according to their flowering time (spring, summer, autumn, late-autumn and winter) that can serve as a useful characterization feature to distinguish Colcicum species. Also, some characters, especially, perigon tube structure, perigon segments surface, perigon tube diameter, flower number, leaf number, leaf hairness, leaf surface, leaf shape were assigned as strong criterion to determine the Colchicum species.Publication Open Access Evaluation of in vitro genotoxic effects induced by in vitro anther culture conditions in sunflower(TAYLOR & FRANCIS INC, 2019-09-02) ALTINKUT UNCUOĞLU, AHU; Cakmak, Elif; Uncuoglu, Ahu Altinkut; Aydin, YildizSunflower is a globally important oilseed, food, and ornamental crop. This study seeks to investigate the genotoxic effects of tissue culture parameters in sunflower calli tissues belongs to two genotypes obtained via anther culture. Anthers were pretreated with cold for 24 hours at 4 degrees C and heat for 2 days at 35 degrees C in the dark and plated onto media supplemented with different concentrations and combinations of 6-benzylaminopurine, 2,4-dichlorophenoxyacetic acid, alpha-naphthalene acetic acid and indole-3-acetic acid. Obtaining calli tissues were used to detect the DNA damage levels by Comet assay, evaluating changes on superoxide dismutase and guaiacol peroxidase activities derived from in vitro culture factors. 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 2 mg/L alpha-naphthalene acetic acid from plant growth regulators showed acute genotoxic effect while 0.5 mg/L indole-3-acetic acid and 0.5 mg/L alpha-naphthalene acetic acid showed no genotoxic effect. Total protein content analysis of antioxidant enzymes revealed that although superoxide dismutase activity did not increase, Guaiacol peroxidase (GPOX) activity decreased in comparison to control. The obtained results have indicated that in vitro culture factors apparently lead to genotoxicity and oxidative stress.Publication Metadata only Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2014) ALTINKUT UNCUOĞLU, AHU; Balta, Handan; Metin, Ozge Karakas; Akfirat, Funda Senturk; Ertugrul, Fahriye; Hasancebi, Semra; Aydin, Yildiz; Akan, Kadir; Mert, Zafer; Turet, Muge; Uncuoglu, Ahu AltinkutAn important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F 2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F 2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC (133bp) marker by the PCR walking method to design SCAR markers for wheat breeding programs.Publication Metadata only Determination of genotoxic effects of organochlorine pesticides in wheat (triticum aestivum l.) by comet assay(Nova, 2019-10-01) AYDİN, YILDIZ; Aydin Y.DETERMINATION of GENOTOXIC EFFECTS of ORGANOCHLORINE PESTICIDES in WHEAT (Triticum aestivum L.) by COMET ASSAYMelek Adiloğlu Öztürk1,Yıldız Aydın1* 1Department of Biology, Faculty of Science and Letters, Marmara University, Istanbul, TurkeyAbstractDue to the detection of pesticides in underground and surface waters in many agricultural regions of the world, the environmental dimension of pesticide use is an important issue discussed today. Continuous use of pesticides in agriculture causes genetic resistance to pests. Pesticides threaten the ecosystem by altering the structure and species distribution of the ecosystem and disrupting the normal balance between food chains and therefore need to be monitored under strict supervision. In this study, our aim was to determine the genotoxic effects of endosulfan (ES) pesticide at different time and concentrations in wheat (Triticum aestivum L.). Leaf samples were taken from two-week old wheat seedlings of the negative control (Hoagland solution), positive control (Hoagland solution containing 0.1% H2O2) and different concentrations (1 g/L, 2 g/L and 4 g/ L) of ES in treated groups at 6h, 12h and 18h and were examined by comet technique (single cell gel electrophoresis).DNA damage levels were found that increase significantly after the ES application to wheat seedling at all the time and the concentrations that evaluated by comet analysis. In wheat seedlings, the highest DNA damage (% Tail DNA:50, Olive tail moment: 0.34) were determined at 12h of 2 g/L ES concentration (p<0.001). While a non-linear decrease with time in the level of DNA damage were detected in all groups at the end of the 6h, 12h, and 18h of ES treatment by comparing with comet data, the DNA damage was increased in 2 g/L ES at 12h (% Tail DNA:50, Olive tail moment: 0.34) comparing with 6h.The obtained results of this study demonstrated that the ES is a genotoxic agent causing DNA breaks in wheat. Also, the results related with decrease in the level of DNA damage obtained from this study will contribute to the determination of the spraying and harvesting time as well as the determination of the appropriate concentrations in the countries using this pesticide. This work was supported by the Research Foundation of Marmara University (BAPKO no. FEN-C-031210-0285). * Corresponding Author Email: ayildiz@marmara.edu.tr.Publication Metadata only Determination of genotoxic effects of boron in wheat (Triticum aestivum L.) by comet assay(ELSEVIER SCIENCE BV, 2012) VARDAR, FİLİZ; Sahin, Ezgi Cabuk; Akgul, Nilufer; Dayi, Seren; Ismailoglu, Isil; Vardar, Filiz; Gulsoy, Nagihan; Aydin, YildizPublication Metadata only Imatinib reduces bone marrow fibrosis and overwhelms the adverse prognostic impact of reticulin formation in patients with chronic myeloid leukaemia(BMJ PUBLISHING GROUP, 2016) AYDİN, YILDIZ; Simsek, Eda Tanrikulu; Eskazan, Ahmet Emre; Cengiz, Mahir; Ar, Muhlis Cem; Ekizoglu, Seda; Salihoglu, Ayse; Gulturk, Emine; Elverdi, Tugrul; Aydin, Seniz Ongoren; Demiroz, Ahu Senem; Buyru, Ayse Nur; Baslar, Zafer; Ozbek, Ugur; Ferhanoglu, Burhan; Aydin, Yildiz; Tuzuner, Nukhet; Soysal, TeomanAims Before the era of tyrosine kinase inhibitors (TKIs), the presence of bone marrow fibrosis (MF) in patients with chronic myeloid leukaemia (CML) has been established as a poor prognostic factor. The aim of the present study was to evaluate the effects of imatinib treatment on MF and the prognostic significance of MF at this new era of CML therapy. Methods The study cohort consisted of 135 patients with CML who were exposed to imatinib. The grades of MF pre and post imatinib together with cytogenetic and molecular responses were evaluated. Results Severe MF (grade II-III) was observed in 44 (33%) patients prior to imatinib therapy, and in 8 (8%) after 12months of imatinib treatment (p=0.001). The complete cytogenetic response (CCyR) rates at 12months did not differ according to the pre-imatinib MF grades, and CCyR rates in patients with grades 0, I, II and III MF were 36/47 (76.5%), 26/33 (78.7%), 12/23 (52.1%) and 7/10 (70%), respectively (p=0.127). There was no significant difference between patients with or without CCyR at 12months of imatinib regarding grades of MF (p=0.785). The distribution of the major molecular response rates at 18months according to pre-treatment grades of MF were determined as grade 0 in 38/45 (84.4%), grade I in 21/28 (75%), grade II in 14/21 (66.6%) and grade III in 7/10 (70%) (p=0.112). There was no significant difference in overall survival rates between initial MF mild (grade 0-I) and severe (grade II-III) groups (p=0.278). Conclusions According to our findings, MF regresses with imatinib therapy over time, and the MF grades at diagnosis do not have a negative impact on the responses to imatinib treatment. Therefore, the adverse prognostic impact of the MF among patients with CML seems to disappear in the era of the TKIs.Publication Open Access SSR Markers Suitable for Marker Assisted Selection in Sunflower for Downy Mildew Resistance(DE GRUYTER POLAND SP ZOO, 2018-09-22) ALTINKUT UNCUOĞLU, AHU; Sahin, Ezgi Cabuk; Kalenderoglu, Aral; Aydin, Yildiz; Evci, Goksel; Uncuoglu, Ahu AltinkutThe effectiveness of Pl genes is known to be resistant to downy mildew (DM) disease affected by fungus Plasmopara halstedii in sunflower. In this study phenotypic analysis was performed using inoculation tests and genotypic analysis were carried out with three DM resistance genes Plarg, Pl13 and Pl8. A total of 69 simple sequence repeat markers and 241 F-2 individuals derived from a cross of RHA-419 (R) x P6LC (S), RHA-419 (R) x CL (S), RHA-419 (R) x OL (S), RHA419 (R) x 9758R (S), HA-R5 (R) x P6LC (S) and HA89 (R) x P6LC (S) parental lines were used to identify resistant hybrids in sunflower. Results of SSR analysis using markers linked with downy mildew resistance genes (Plarg, Pl8 and Pl13) and downy mildew inoculation tests were evaluated together and ORS716 (for Plarg and Pl13), HA4011 (for Pl8) markers showed positive correlation with their phenotypic results. These results suggest that these markers are associated with DM resistance and they can be used successfully in marker-assisted selection for sunflower breeding programs specific for downy mildew resistance.Publication Open Access CRISPR/Cas9-mediated targeted mutagenesis of FAD2-1 gene for oleic acids composition in sunflower(2022-12-01) AYDİN, YILDIZ; ALTINKUT UNCUOĞLU, AHU; Uslu Y. Z., Ural B. N., Cebrailoglu N., AYDİN Y., ÖZDEN ÇİFTÇİ Y., ALTINKUT UNCUOĞLU A.Sunflower (Helianthus annuus L.) is the most widespread plant used for production of oil among all oilseeds cultivated in Turkey in terms of agricultural area and production. Sunflower with high oleic acid content is always desirable because of benefits on health and industrial use. The enzyme FAD2-1 catalyzes the conversion of oleic acid to linoleic acid in sunflower. The fatty acid composition can be enhanced by gene editing of FAD2-1 gene by CRISPR/Cas9 technique which has been applied recently as a new breeding technique to improve productivity and enhance sustainability in agriculture. Hence, CRISPR/Cas9 genome editing systems were utilized to knockout the FAD2-1 gene in order to increase production of oleic acid content. For this purpose, two low oleic sunflower genotypes were transformed with two sgRNA that target FAD2-1 gene. sgRNA expression cassettes were assembled into the binary vector by Golden Gate assembly in a single reaction which is followed by A. tumefaciens-mediated transformation and in vitro germination. Putatively transformed shoots were selected with A. tumefaciens-mediated the optimized kanamycin concentration (100 mg/L) in the medium. The challenges of transformation of sunflower were summarized and possible solutions were proposed. This study indicated that sunflower still can be modified by CRISPR/Cas9 genome editing system to develop high oleic sunflower.Publication Metadata only Clustering of SSR markers located on 2AL for yellow rust resistance in hexaploid wheat (Triticum aestivum L.)(ELSEVIER SCIENCE BV, 2012) AYDİN, YILDIZ; Akfirat, Funda Senturk; Ertugrul, Fahriye; Cakir, Mehmet; Hasancebi, Semra; Aydin, Yildiz; Akan, Kadir; Mert, Zafer; Uncuoglu, Ahu Altinkut