Publication: Evaluating the effects of different remineralization agents on initial enamel lesions in vitro
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Date
2022-04-23
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Abstract
Aim: Disruption of the demineralization–remineralization balance
could activate the development of initial enamel lesions. Extrinsic
assistance is needed to remineralize enamel lesions. The aim of this
study was to evaluate the efects of fuoride varnish, enamel matrix
proteins derivative, and experimental bioactive glass (BAG) on remineralization of initial enamel lesions in vitro.
Materials and Methods: Artifcial initial caries lesions were developed on ffty human enamel samples using demineralization solution (pH 4.5, 37°C, 96 hours). Samples were randomly assigned to 5
groups (n=10): I-Fluoride varnish (Enamelast), II-Experimental BAG
+ 37% phosphoric acid (PA), III-Enamel matrix proteins derivative
(EMPD, Emdogain) +Ethylenediamine-Tetraacetic-Acid (EDTA), IVEMPD+37% PA, V-Control (untreated). All remineralization agents
were applied with pH cycling for 7 days. The samples were scanned by
optical coherence tomography (OCT) at baseline, demineralization, and
after pH cycling. Lesion depths were measured using image analysis
software (ImageJ). Lesions were evaluated using surface microhardness
(SMH) and two fuorescence methods (FluoreCam® and DIAGNOdent
Pen (DDPen)). The data were statistically analyzed by Kruskal Wallis,
Friedman, and Wilcoxon tests (p<0.05).
Results: According to OCT results, fuoride varnish was found to be
the most efective agent in reducing lesion depth (p=0.005). Depending on SMH results, all agents increased the surface hardness values
after pH cycling. No signifcant diference was found among fuoride
varnish, BAG, and EMPD+PA groups. And these microhardness values were signifcantly higher than EMPD+EDTA and control groups
(p<0.001). According to the DDPen score criteria, all groups showed
lower scores compared with the control group (p<0.001), however,
Clinical Oral Investigations (2022) 26:7299–7348 7317
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no signifcant diference was found among the remineralization agent
groups. In FluoreCam assessment, size and intensity values of all
treated groups showed improvement. However, there was no signifcant
diference between the treatment groups in terms of FluoreCam-size
measurements (p=0.186).
Conclusion: BAG and EMPD+PA has a remineralization capacity as
much as fuoride varnish. EMPD+PA showed better microhardness and
lesion intensity results than EMPD+EDTA.
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Keywords
DİŞ HEKİMLİĞİ, ORAL CERRAHİ VE TIP, Klinik Tıp, Klinik Tıp (MED), DENTISTRY, ORAL SURGERY & MEDICINE, CLINICAL MEDICINE, Clinical Medicine (MED), remineralization, bioactive glass, enamel matrix proteins, optical coherence tomography, fuorescence
Citation
SARIALİOĞLU GÜNGÖR A., DALKILIÇ E., ALKAN E., YILMAZ ATALI P., TAĞTEKİN D., \"Evaluating the Effects of Different Remineralization Agents on Initial Enamel Lesions In Vitro\", 11th ConsEuro Congress, Antalya, Türkiye, 21 - 23 Nisan 2022, ss.19-20