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Using proteomics, q-PCR and biochemical methods complementing as a multiapproach to elicit the crucial responses of zebrafish liver exposed to neonicotinoid pesticide

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dc.contributor.authorSESAL, NÜZHET CENK
dc.contributor.authorGÖKALSIN, BARIŞ
dc.contributor.authorKAYHAN, FİGEN ESİN
dc.contributor.authorsKızılkaya Ş., Akpınar G., Sesal N. C., Kasap M., Gökalsın B., Kayhan F. E.
dc.date.accessioned2023-06-22T07:57:14Z
dc.date.available2023-06-22T07:57:14Z
dc.date.issued2023-06-01
dc.description.abstractPesticides enter the environment through runoff and leaching and this raises public concern about effects on non-target organisms. Imidacloprid (IMI) a synthetic pesticide, has an unstable half-life, metabolized in minutes to weeks in the water. To evaluate the effects of IMI on the zebrafish liver, we conducted proteomic, molecular and biochemical analysis in a multi-level approach, to highlight the complementary features regarding the results of each method. Adult zebrafish were exposed to 60 mg/L IMI for 48 hours and were evaluated using nLC-MS/MS for proteins, q-PCR analysis for expression of cat, gpx, pxr, ache, along with CAT and AChE enzyme activities and GSH and MDA assays. Based on proteomics, the regulation of antioxidant and immune responses, as well as gene transcription were significant processes affected. Apoptosis and ER stress pathways were upregulated and there was a down-regulation of cat and gpx genes. There was also elevated CAT activity and GSH and decreased MDA. Additionally, elevated AChE activity and up regulation of ache expression was observed. The multi-approach results included regulators of antioxidant, xenobiotic response and neuro-protective related proteins (genes and enzymes), which overall reflected harmful effects of IMI. Consequently, this study highlights the effects of IMI on zebrafish liver and reveals new potential biomarkers. In this respect, evaluated outcomes reveal the complementary features emphasizing the importance of studying chemicals using several methods. Our study provides deeper insights for future work in ecotoxicological studies regarding IMI and contribute to existing toxicity literature.
dc.identifier.citationKızılkaya Ş., Akpınar G., Sesal N. C., Kasap M., Gökalsın B., Kayhan F. E., "Using proteomics, q-PCR and biochemical methods complementing as a multiapproach to elicit the crucial responses of zebrafish liver exposed to neonicotinoid pesticide", COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY - PART D: GENOMICS AND PROTEOMICS, cilt.101103, sa.101103, ss.101103, 2023
dc.identifier.doi10.1016/j.cbd.2023.101103
dc.identifier.endpage101103
dc.identifier.issn1744-117X
dc.identifier.issue101103
dc.identifier.startpage101103
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1744117X23000485?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11424/290511
dc.identifier.volume101103
dc.language.isoeng
dc.relation.ispartofCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY - PART D: GENOMICS AND PROTEOMICS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectYaşam Bilimleri
dc.subjectHidrobiyoloji
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectTemel Bilimler
dc.subjectLife Sciences
dc.subjectHydrobiology
dc.subjectMolecular Biology and Genetics
dc.subjectNatural Sciences
dc.subjectTemel Bilimler (SCI)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectDoğa Bilimleri Genel
dc.subjectBiyoloji ve Biyokimya
dc.subjectÇOK DİSİPLİNLİ BİLİMLER
dc.subjectBİYOLOJİ
dc.subjectNatural Sciences (SCI)
dc.subjectLife Sciences (LIFE)
dc.subjectNATURAL SCIENCES, GENERAL
dc.subjectBIOLOGY & BIOCHEMISTRY
dc.subjectMOLECULAR BIOLOGY & GENETICS
dc.subjectMULTIDISCIPLINARY SCIENCES
dc.subjectBIOLOGY
dc.subjectMoleküler Biyoloji
dc.subjectMultidisipliner
dc.subjectMolecular Biology
dc.subjectMultidisciplinary
dc.titleUsing proteomics, q-PCR and biochemical methods complementing as a multiapproach to elicit the crucial responses of zebrafish liver exposed to neonicotinoid pesticide
dc.typearticle
dspace.entity.typePublication
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