Publication: Protective Effects of Momordica charantia (Bitter Melon) against Metho-trexate-induced Kidney Damage
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2023-01-01
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Abstract
Background: Methotrexate is a cytotoxic chemotherapeutic agent that has severe side ef-fects, such as nephrotoxicity. Momordica charantia is a bright yellow-orange fruity plant that has been shown to have antioxidant, antidiabetic, and anti-inflammatory properties. Methods: 24 Sprague Dawley male rats were divided into three experimental groups (8 rats in each): Control (C); Methotrexate (MTX); and Methotrexate plus Momordica charantia (MTX+MC). All rats were fed ad libitum and tap water. Methotrexate was administered at 20 mg/kg intraperitoneally as a single dose. In the MTX+MC group, MC was administered at a dose of 50mg/kg for 5 days orally. At the end of the 5th day, the rats were decapitated and kidney samples were taken to analyze glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), 8-hydroxy-2\"-deoxyguanosine (8-OHdG) and caspase-3 activity. Data was analyzed with GraphPad Prism 5.0. Results: Findings showed that while there was a significant increase in MDA, MPO, 8-OHdG levels, and an essential reduction in GSH levels in the MTX-treated group when compared with the control group, bitter melon treatment significantly reversed MDA, MPO, and 8-OHdG levels (p< 0.001). GSH level elevation was observed in the MTX-MC group when compared to the MTX-treated group (p< 0.001). Conclusion: This study showed that bitter melon is thought to have a protective effect against kidney damage caused by methotrexate. With future studies, we believe that the use of bitter melon extract as a protective agent in kidney damage caused by drug-induced oxidative damage will bring an innova-tive approach to treatment.
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Temel Eczacılık Bilimleri, Eczacılık, Yaşam Bilimleri, Sağlık Bilimleri, Temel Bilimler, Basic Pharmaceutics Sciences, Pharmacology and Therapeutics, Life Sciences, Health Sciences, Natural Sciences, Yaşam Bilimleri (LIFE), Farmakoloji ve Toksikoloji, FARMAKOLOJİ VE ECZACILIK, Life Sciences (LIFE), PHARMACOLOGY & TOXICOLOGY, PHARMACOLOGY & PHARMACY, Genel Farmakoloji, Toksikoloji ve Eczacılık, Farmakoloji (tıbbi), General Pharmacology, Toxicology and Pharmaceutics, Pharmacology (medical), kidney, methotrexate, Momordica charantia, nephrotoxicity, oxidative damage, protective effect
Citation
MACİT Ç., ÖZBEYLİ D., Cevik O., Cetin M., Sener G., Özkan S., "Protective Effects of Momordica charantia (Bitter Melon) against Metho-trexate-induced Kidney Damage", Current Drug Therapy, cilt.18, sa.3, ss.231-236, 2023