Publication: Effect of the use of remineralization agents before resin infiltration on the treatment of initial enamel lesions: OCT, ultrasound, and SEM evaluation
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Date
2023-06-11
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Abstract
Aim: It was aimed to investigate the effect of using remineralizing agent before resin
infiltration application on the treatment of initial enamel lesions with OCT (Optical Coherence
Tomography), Ultrasound and SEM (Scanning Electromicroscope).
Materials and Methods: 80 enamel samples were kept in the demineralization solution (pH
4.5, 37°C) for 96 hours. Demineralized enamel samples will be randomly divided into 8
groups (n=10) including a remineralizing agent(Toothmousse/GC, Remin Pro/VOCO, Medical
Mineral Gel/ROCS), resin infiltration (ICON/DMG), and a combined treatment of both. All
remineralization agents were applied in remineralization cycle 2 times a day for 1
week.Measurements made from the enamel samples at the baseline, after demineralization
and remineralization procedures evaluated with the OCT (Topcon3D OCT-1 Maestro) and
ultrasonic system (Novascope 4500:NDT) lesion depths were measured. The sample
surfaces were examined under SEM/EDS (Zeiss EVO-MA10) at x1000, 2500, 5000 and
10000. Data were analyzed in R program with WRS2. Two-way Robust Anova, Benferroni
Test, and ICC (Intra-Class Correlation Coefficient) was used to examine the agreement
between measurements. Significance level was set at p<0.05.
Results: There was no statistical difference between the groups in OCT (p=0.884) and
Ultrasound (p=0.051) median values after the protocols. In both measurement methods, the
effect of remineralization treatments was statistically significant for lession depth (p<0.01).
OCT median values were 104.01 in baseline, 144.78 in post-demineralization, and 90.5 in
post-remineralization. Ultrasound median values were 1.34 in baseline, 1.1 in postdemineralization, and 2.36 inpost-remineralization. The highest ultrasound median value was
obtained in the Remin Pro+ICON after remineralization (2.67), while the lowest value was
obtained after demineralization (1.04). As a result of EDS analysis, the lowest Ca ratio
(%atomic) was observed on the grup 5-ICON applied enamel surface (9.35).
Conclusion: Although there is no significant difference between the groups, all groups were
found efficient for remineralization treatments according to OCT, Ultrasound, and SEM/EDS
analysis.
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Keywords
remineralization, demineralization, resin infiltration technique, remineralization agent, pH cycle
Citation
MANAV ÖZEN A., DOĞU KAYA B., YILMAZ ATALI P., SARIALİOĞLU GÜNGÖR A., ALKAN E., DALKILIÇ E., TAĞTEKİN D., TÜRKMEN C., \"Effect of the Use of Remineralization Agents Before Resin Infiltration on the Treatment of Initial Enamel Lesions: OCT, Ultrasound, and SEM Evaluation\", IV. PREVENT FROM CARIES SYMPOSIUM, İstanbul, Türkiye, 08 Mayıs 2023, ss.84